ArticleAssay and drug development technologies2012
Domain-based biosensor assay to screen for epidermal growth factor receptor modulators in live cells.
Article in Assay and drug development technologies, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
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- Discovering common pathogenetic processes between COVID-19 and diabetes mellitus by differential gene expression pattern analysis.Briefings in bioinformatics · 2021Article
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation.Science signaling · 2019Article
- Piperonylic acid stimulates keratinocyte growth and survival by activating epidermal growth factor receptor (EGFR).Scientific reports · 2018Article
- Systematic Identification of Oncogenic EGFR Interaction Partners.Journal of molecular biology · 2017Article
- ABCG2 regulates self-renewal and stem cell marker expression but not tumorigenicity or radiation resistance of glioma cells.Scientific reports · 2016Article
- Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1.Advances in biological regulation · 2016Article
- EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy.PloS one · 2016Article
- Chemical & RNAi screening at MSKCC: a collaborative platform to discover & repurpose drugs to fight disease.Combinatorial chemistry & high throughput screening · 2014Article
- Article
- An arrayed genome-scale lentiviral-enabled short hairpin RNA screen identifies lethal and rescuer gene candidates.Assay and drug development technologies · 2013Article
- Phosphotyrosine recognition domains: the typical, the atypical and the versatile.Cell communication and signaling : CCS · 2012Article
- A high-content imaging workflow to study Grb2 signaling complexes by expression cloning.Journal of visualized experiments : JoVE · 2012Article
- Designs and concept reliance of a fully automated high-content screening platform.Journal of laboratory automation · 2012Article
- A high-content biosensor-based screen identifies cell-permeable activators and inhibitors of EGFR function: implications in drug discovery.Journal of biomolecular screening · 2012Article
- An image-based biosensor assay strategy to screen for modulators of the microRNA 21 biogenesis pathway.Combinatorial chemistry & high throughput screening · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Traditional drug discovery efforts have resulted in the approval of a handful of receptor tyrosine kinase (RTK) inhibitors; however, their discovery relied solely on screening recombinant kinases, often with poor cellular activity outcome. The ability to screen RTKs in their natural environment is sought as an alternative approach. We have adapted a novel strategy utilizing a green fluorescent protein-labeled SRC homology 2 domain-based biosensor as a surrogate reporter of endogenous epidermal growth factor receptor (EGFR) activity in A549 cells. Upon activation of the receptor, EGFR function in live cells is measured by the number of green granules that form. Here we describe assay miniaturization and demonstrate specificity for EGFR through its chemical inhibition and RNAi-dependent knockdown resulting in complete abrogation of granule formation. Gefitinib and PD 153035 were identified as hits in a pilot screen. This approach allows for the identification of novel EGFR modulators in high-throughput formats for screening chemical and RNAi libraries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.