ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Concentration-dependent requirement for local protein synthesis in motor neuron subtype-specific response to axon guidance cues.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed.
- Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.Nature communications · 2022Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- The Geometry of Limb Motor Innervation is Controlled by the Dorsal-Ventral Compartment Boundary in the Chick Limbless Mutant.Neuroscience · 2020Article
- Anterograde Axonal Transport in Neuronal Homeostasis and Disease.Frontiers in molecular neuroscience · 2020Review
- Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.eLife · 2019Article
- High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients.Nature communications · 2018Article
- Axon guidance pathways and the control of gene expression.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Review
- Demonstration of ion channel synthesis by isolated squid giant axon provides functional evidence for localized axonal membrane protein translation.Scientific reports · 2018Article
- Sonic Hedgehog Guides Axons via Zipcode Binding Protein 1-Mediated Local Translation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Intra-axonal protein synthesis in development and beyond.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2016Review
- Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units.Science advances · 2016Article
- Regulation of neuronal gene expression by local axonal translation.Current genetic medicine reports · 2016Article
- Compartmentalized Platforms for Neuro-Pharmacological Research.Current neuropharmacology · 2016Review
- Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex.Nature communications · 2015Article
- Coupled local translation and degradation regulate growth cone collapse.Nature communications · 2015Article
- Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones.Neural development · 2015Article
- Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.Nature biotechnology · 2015Article
- Local translation of TC10 is required for membrane expansion during axon outgrowth.Nature communications · 2014Article
- Label-free characterization of emerging human neuronal networks.Scientific reports · 2014Article
- RNA-binding protein Vg1RBP regulates terminal arbor formation but not long-range axon navigation in the developing visual system.Developmental neurobiology · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Formation of functional motor circuits relies on the ability of distinct spinal motor neuron subtypes to project their axons with high precision to appropriate muscle targets. While guidance cues contributing to motor axon pathfinding have been identified, the intracellular pathways underlying subtype-specific responses to these cues remain poorly understood. In particular, it remains controversial whether responses to axon guidance cues depend on axonal protein synthesis. Using a growth cone collapse assay, we demonstrate that mouse embryonic stem cell-derived spinal motor neurons (ES-MNs) respond to ephrin-A5, Sema3f, and Sema3a in a concentration-dependent manner. At low doses, ES-MNs exhibit segmental or subtype-specific responses, while this selectivity is lost at higher concentrations. Response to high doses of semaphorins and to all doses of ephrin-A5 is protein synthesis independent. In contrast, using microfluidic devices and stripe assays, we show that growth cone collapse and guidance at low concentrations of semaphorins rely on local protein synthesis in the axonal compartment. Similar bimodal response to low and high concentrations of guidance cues is observed in human ES-MNs, pointing to a general mechanism by which neurons increase their repertoire of responses to the limited set of guidance cues involved in neural circuit formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.