ArticleProceedings. Biological sciences2012
Reduced metabolic rate and oxygen radicals production in stored insect sperm.
Article in Proceedings. Biological sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Per capita sperm metabolism is density dependent.The Journal of experimental biology · 2024Pooled it
- Sperm storage causes sperm senescence in human and non-human animals.Proceedings. Biological sciences · 2026Article
- Proteome Analysis of Spermathecal Fluid and Seminal Plasma Reveals the Mechanism of Sperm Storage inAnimals : an open access journal from MDPI · 2025Article
- Mating-induced patterns of spermathecal fluid protein expression in two eusocial insect species, Lasius niger and Apis mellifera.Scientific reports · 2025Article
- Drosophila melanogaster sperm turn more oxidative in the female.The Journal of experimental biology · 2024Article
- Paternal environment effects are driven by female reproductive fluid but not sperm age in an external fertilizer.Biology letters · 2023Article
- Patterns of Remating Behaviour inFrontiers in physiology · 2022Article
- Article
- Production and scavenging of reactive oxygen species both affect reproductive success in male and female Drosophila melanogaster.Biogerontology · 2021Article
- Upregulation of Transferrin and Major Royal Jelly Proteins in the Spermathecal Fluid of Mated Honeybee (Insects · 2021Article
- The role of oxidative stress in postcopulatory selection.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Metabolite Support of Long-Term Storage of Sperm in the Spermatheca of Honeybee (Frontiers in physiology · 2020Article
- Distinct metabolic profiles in Drosophila sperm and somatic tissues revealed by two-photon NAD(P)H and FAD autofluorescence lifetime imaging.Scientific reports · 2019Article
- The deteriorating soma and the indispensable germline: gamete senescence and offspring fitness.Proceedings. Biological sciences · 2019Article
- The importance of a halotyrosine dehalogenase forThe Journal of biological chemistry · 2018Article
- Rival seminal fluid induces enhanced sperm motility in a polyandrous ant.BMC evolutionary biology · 2018Article
- Article
- Sneaker Male Squid Produce Long-lived Spermatozoa by Modulating Their Energy Metabolism.The Journal of biological chemistry · 2016Article
- Oxidative stress does not play a primary role in the toxicity induced with clinical doses of doxorubicin in myocardial H9c2 cells.Molecular and cellular biochemistry · 2016Article
- Multiple Mating, Paternity and Complex Fertilisation Patterns in the Chokka Squid Loligo reynaudii.PloS one · 2016Article
Corrections and comments
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Females of internally fertilizing species can significantly extend sperm lifespan and functionality during sperm storage. The mechanisms for such delayed cellular senescence remain unknown. Here, we apply current hypotheses of cellular senescence developed for diploid cells to sperm cells, and empirically test opposing predictions on the relationship between sperm metabolic rate and oxygen radical production in an insect model, the cricket Gryllus bimaculatus. Using time-resolved microfluorimetry, we found a negative correlation between metabolic rate (proportion of protein-bound NAD[P]H) and in situ intracellular oxygen radicals production in freshly ejaculated sperm. In contrast, sperm stored by females for periods of 1 h to 26 days showed a positive correlation between metabolic rate and oxygen radicals production. At the same time, stored sperm showed a 37 per cent reduced metabolic rate, and 42 per cent reduced reactive oxygen species (ROS) production, compared with freshly ejaculated sperm. Rank differences between males in ROS production and metabolic rate observed in ejaculated sperm did not predict rank differences in stored sperm. Our method of simultaneously measuring ROS production and metabolic rate of the same sample has the advantage of providing data that are independent of sperm density and any extracellular antioxidants that are proteins. Our method also excludes effects owing to accumulated hydrogen peroxide. Our results unify aspects of competing theories of cellular ageing and suggest that reducing metabolic rate may be an important means of extending stored sperm lifespan and functionality in crickets. Our data also provide a possible explanation for why traits of ejaculates sampled from the male may be rather poor predictors of paternity in sexual selection studies and likelihood of pregnancy in reproductive medicine.
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