ArticleCytoskeleton (Hoboken, N.J.)2012
Multiple domains of human CLASP contribute to microtubule dynamics and organization in vitro and in Xenopus egg extracts.
Article in Cytoskeleton (Hoboken, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- Microtubule-Associated Proteins: From Dynamic Regulation of Microtubules to Cellular Architecture.Cells · 2026Review
- α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.Nature communications · 2024Article
- CLASP-mediated competitive binding in protein condensates directs microtubule growth.Nature communications · 2024Article
- CLASP2 facilitates dynamic actin filament organization along the microtubule lattice.Molecular biology of the cell · 2023Article
- Article
- C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.PLoS genetics · 2020Article
- Leaving no-one behind: how CENP-E facilitates chromosome alignment.Essays in biochemistry · 2020Review
- CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments.The Journal of cell biology · 2020Article
- Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-end Tracking Protein Network in Adipocytes.Molecular & cellular proteomics : MCP · 2019Article
- CLASP Facilitates Transitions between Cortical Microtubule Array Patterns.Plant physiology · 2018Article
- An isolated CLASP TOG domain suppresses microtubule catastrophe and promotes rescue.Molecular biology of the cell · 2018Article
- Human CLASP2 specifically regulates microtubule catastrophe and rescue.Molecular biology of the cell · 2018Article
- Phosphorylation Dynamics Dominate the Regulated Proteome during Early Xenopus Development.Scientific reports · 2017Article
- Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry.Scientific reports · 2017Article
- Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics.Scientific reports · 2017Article
- GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics.Journal of cell science · 2017Article
- Article
- A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends.The Journal of cell biology · 2016Article
- Crescerin uses a TOG domain array to regulate microtubules in the primary cilium.Molecular biology of the cell · 2015Article
- TPX2 phosphorylation maintains metaphase spindle length by regulating microtubule flux.The Journal of cell biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic linker associated proteins (CLASPs) comprise a class of microtubule (MT) plus end-binding proteins (+TIPs) that contribute to the dynamics and organization of MTs during many cellular processes, among them mitosis. Human CLASP proteins contain multiple MT-binding domains, including tumor over-expressed gene (TOG) domains, and a Ser-x-Ile-Pro (SxIP) motif known to target some +TIPs though interaction with end-binding protein 1 (EB1). However, how individual domains contribute to CLASP function is poorly understood. We generated full-length recombinant human CLASP1 and a series of truncation mutants and found that two N-terminal TOG domains make the strongest contribution to MT polymerization and bundling, but also identified a third TOG domain that further contributes to CLASP activity. Plus end tracking by CLASP requires the SxIP motif and interaction with EB1. The C-terminal coiled-coil domain mediates dimerization and association with many other factors, including the kinetochore motor centromere protein E (CENP-E), and the chromokinesin Xkid. Only the full-length protein was able to rescue spindle assembly in Xenopus egg extracts depleted of endogenous CLASP. Deletion of the C-terminal domain caused aberrant MT polymerization and dramatic spindle phenotypes, even with small amounts of added protein, indicating that proper localization of CLASP activity is essential to control MT polymerization during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.