ArticleNucleic acids research2012
Amplified microRNA detection by templated chemistry.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Chemical autoligation with phosphorothioate- and sulfonamide-terminated DNA via intramolecular cross-activation.Communications chemistry · 2025Article
- Entropy-Driven Molecular Beacon Assisted Special RCA Assay with Enhanced Sensitivity for Room Temperature DNA Biosensing.Biosensors · 2024Article
- Programmable design of isothermal nucleic acid diagnostic assays through abstraction-based models.Nature communications · 2022Article
- Bioorthogonal Reactions of Triarylphosphines and Related Analogues.Chemical reviews · 2021Review
- Bioorthogonal Ligations and Cleavages in Chemical Biology.ChemistryOpen · 2020Review
- Isothermal digital detection of microRNAs using background-free molecular circuit.Science advances · 2020Article
- High-Fidelity Nanopore Sequencing of Ultra-Short DNA Targets.Analytical chemistry · 2019Article
- Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs.Chemical science · 2018Article
- Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes.Chemical science · 2018Article
- Quantification of microRNAs directly from body fluids using a base-stacking isothermal amplification method in a point-of-care device.Biomedical microdevices · 2017Article
- Subnanomolar Detection of Oligonucleotides through Templated Fluorogenic Reaction in Hydrogels: Controlling Diffusion to Improve Sensitivity.Angewandte Chemie (International ed. in English) · 2017Article
- Fluorogenic Templated Reaction Cascades for RNA Detection.Journal of the American Chemical Society · 2017Article
- The Discovery of Rolling Circle Amplification and Rolling Circle Transcription.Accounts of chemical research · 2016Article
- ATP-Releasing Nucleotides: Linking DNA Synthesis to Luciferase Signaling.Angewandte Chemie (International ed. in English) · 2016Article
- Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA.Chemical science · 2015Article
- Bioorthogonal tetrazine-mediated transfer reactions facilitate reaction turnover in nucleic acid-templated detection of microRNA.Journal of the American Chemical Society · 2014Article
- Noninvasive micromarkers.Clinical chemistry · 2014Review
- Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides.Nucleic acids research · 2013Article
- Review
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Authors and funding
2 authors.
Funding
Abstract
MicroRNAs (miRNAs) are a class of RNAs that play important regulatory roles in the cell. The detection of microRNA has attracted significant interest recently, as abnormal miRNA expression has been linked to cancer and other diseases. Here, we present a straightforward method for isothermal amplified detection of miRNA that involves two separate nucleic acid-templated chemistry steps. The miRNA first templates the cyclization of an oligodeoxynucleotide from a linear precursor containing a 5'-iodide and a 3'-phosphorothioate. The sequence is amplified through rolling circle amplification with 29 DNA polymerase and then detected via a second amplification using fluorogenic templated probes. Tests showed that the cyclization proceeds in ∼50% yield over 24 h and is compatible with the conditions required for rolling circle polymerization, unlike enzymatic ligations which required non-compatible buffer conditions. The polymerization yielded 188-fold amplification, and separate experiments showed ∼15-fold signal amplification from the templated fluorogenic probes. When all components are combined, results show miRNA detection down to 200 pM in solution, and correlation of the detected signal with the initial concentration of miRNA. The doubly templated double-amplification method demonstrates a new approach to detection of rolling circle products and significant advantages in ease of operation for miRNA detection.
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Registered trials
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