ArticleGene therapy2012
Reprogramming axonal behavior by axon-specific viral transduction.
Article in Gene therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Heart-Specific Spinal and Vagal Afferents: Transcriptomic Signatures and Optogenetically Modulated Functional Coupling With Cardiomyocytes.Comprehensive Physiology · 2026Article
- KHSRP-mediated decay of axonally localized prenyl-Cdc42 mRNA slows nerve regeneration.PLoS genetics · 2025Article
- Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Targeting chondroitinase ABC to axons enhances the ability of chondroitinase to promote neurite outgrowth and sprouting.PloS one · 2020Article
- Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala.eLife · 2019Article
- Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons.Cell reports · 2019Article
- Local translation in neurons: visualization and function.Nature structural & molecular biology · 2019Review
- Axonal localization of neuritin/CPG15 mRNA is limited by competition for HuD binding.Journal of cell science · 2017Article
- A Staufen1-mediated decay pathway influences the local transcriptome in axons.Translation (Austin, Tex.) · 2017Article
- Protein synthetic machinery and mRNA in regenerating tips of spinal cord axons in lamprey.The Journal of comparative neurology · 2016Article
- Intra-axonal protein synthesis - a new target for neural repair?Neural regeneration research · 2016Review
- Article
- mRNAs and Protein Synthetic Machinery Localize into Regenerating Spinal Cord Axons When They Are Provided a Substrate That Supports Growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Targeting axonal protein synthesis in neuroregeneration and degeneration.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2015Review
- Molecular determinants of the axonal mRNA transcriptome.Developmental neurobiology · 2014Review
- Axonal transcription factors: novel regulators of growth cone-to-nucleus signaling.Developmental neurobiology · 2014Review
- Peripheral nerve axons contain machinery for co-translational secretion of axonally-generated proteins.Neuroscience bulletin · 2013Article
- Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors.Frontiers in cellular neuroscience · 2013Article
- Intra-axonal translation of RhoA promotes axon growth inhibition by CSPG.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Insights into the roles of local translation from the axonal transcriptome.Open biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The treatment of axonal disorders, such as diseases associated with axonal injury and degeneration, is limited by the inability to directly target therapeutic protein expression to injured axons. Current gene therapy approaches rely on infection and transcription of viral genes in the cell body. Here, we describe an approach to target gene expression selectively to axons. Using a genetically engineered mouse containing epitope-labeled ribosomes, we find that neurons in adult animals contain ribosomes in distal axons. To use axonal ribosomes to alter local protein expression, we utilized a Sindbis virus containing an RNA genome that has been modified so that it can be directly used as a template for translation. Selective application of this virus to axons leads to local translation of heterologous proteins. Furthermore, we demonstrate that selective axonal protein expression can be used to modify axonal signaling in cultured neurons, enabling axons to grow over inhibitory substrates typically encountered following axonal injury. We also show that this viral approach also can be used to achieve heterologous expression in axons of living animals, indicating that this approach can be used to alter the axonal proteome in vivo. Together, these data identify a novel strategy to manipulate protein expression in axons, and provides a novel approach for using gene therapies for disorders of axonal function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.