ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012
Application of Legionella pneumophila-specific quantitative real-time PCR combined with direct amplification and sequence-based typing in the diagnosis and epidemiological investigation of Legionnaires' disease.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Therapeutical strategies in cavitary legionnaires' disease, two cases from the field and a systematic review.Annals of clinical microbiology and antimicrobials · 2023Pooled it
- Review
- Identification rate ofJournal of clinical microbiology · 2024Article
- Severe Legionnaires' disease.Annals of intensive care · 2024Review
- Hyper-inflammatory profile and immunoparalysis in patients with severe Legionnaires' disease.Frontiers in cellular and infection microbiology · 2023Article
- Detection of Diverse Sequence Types of Legionella pneumophila by Legiolert Enzymatic-Based Assay and the Development of a Long-Term Storage Protocol.Microbiology spectrum · 2022Article
- Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing.Microbiology spectrum · 2022Article
- Dynamics of Legionella Community Interactions in Response to Temperature and Disinfection Treatment: 7 Years of Investigation.Microbial ecology · 2022Article
- Non-IDCases · 2022Article
- New Insight regardingMicrobiology spectrum · 2021Article
- Occurrence ofPathogens (Basel, Switzerland) · 2021Article
- How Molecular Typing Can SupportInternational journal of environmental research and public health · 2020Article
- The Role of Sensor-Activated Faucets in Surgical Handwashing Environment as a Reservoir ofPathogens (Basel, Switzerland) · 2020Article
- Evaluation of MALDI-TOF Mass Spectrometry in Diagnostic and Environmental Surveillance ofFrontiers in microbiology · 2020Article
- Sit bath systems: A new source of Legionella infection.PloS one · 2020Article
- Advances inPathogens (Basel, Switzerland) · 2019Article
- Legionella SBT applied directly to respiratory samples as a rapid molecular epidemiological tool.Scientific reports · 2019Article
- Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem.Epidemiology and infection · 2018Article
- Low genomic diversity of Legionella pneumophila within clinical specimens.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2018Article
- Epidemiological information is key when interpreting whole genome sequence data - lessons learned from a largeEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of Legionella pneumophila DNA in clinical specimens using quantitative real-time polymerase chain reaction (qPCR) combined with direct sequence-based typing (SBT) offers rapid confirmation and timely intervention in the investigation of cases of Legionnaires' disease (LD). We assessed the utility of a specific L. pneumophila qPCR assay targeting the macrophage infectivity potentiator (mip) gene and internal process control with three clinical specimen types from confirmed LD cases. The assay was completely specific for L. pneumophila, as demonstrated by positive results for 39/39 strains from all subspecies and 16 serogroups. No cross-reaction was observed with any of the 54 Legionella non-pneumophila (0/69 strains) or 21 non-Legionella (0/58 strains). All L. pneumophila culture-positive respiratory samples (81/81) were qPCR-positive. Of 80 culture-negative samples tested, 47 (58.8%) were qPCR-positive and none were inhibitory. PCR was significantly more sensitive than culture for samples taken ≤ 2 days of hospitalisation (94.7% vs. 79.6%), with the difference being even more marked for samples taken between 3 and 14 days (79.3% vs. 47.8%). Overall, the sensitivity of the qPCR was ∼30% greater than that of culture and direct typing on culture-negative PCR-positive samples resulted in full 7-allele profiles from 23/46, 5 to 6 alleles from 8/46 and ≥ 1 allele from 43/46 strains.
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