ArticleAmerican journal of physiology. Cell physiology2012
Breast cancer cell-derived matrix supports vascular morphogenesis.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Advances in cell-derived extracellular matrix for 3D tumor modelling.Materials today. Bio · 2026Review
- Multimodal mechano-microscopy reveals mechanical phenotypes of breast cancer spheroids in three dimensions.APL bioengineering · 2024Article
- Elastin-like Recombinamer Hydrogels as Platforms for Breast Cancer Modeling.Biomacromolecules · 2023Article
- Heterotypic Tumor Spheroids in Agitation-Based Cultures: A Scaffold-Free Cell Model That Sustains Long-Term Survival of Endothelial Cells.Frontiers in bioengineering and biotechnology · 2021Article
- Hypoxia in Breast Cancer-Scientific Translation to Therapeutic and Diagnostic Clinical Applications.Frontiers in oncology · 2021Review
- Engineering Breast Cancer Microenvironments and 3D Bioprinting.Frontiers in bioengineering and biotechnology · 2018Review
- Aligned fibers direct collective cell migration to engineer closing and nonclosing wound gaps.Molecular biology of the cell · 2017Article
- Loss of caveolin-1 alters extracellular matrix protein expression and ductal architecture in murine mammary glands.PloS one · 2017Article
- The Influence of Platelet-Derived Growth Factor and Bone Morphogenetic Protein Presentation on Tubule Organization by Human Umbilical Vascular Endothelial Cells and Human Mesenchymal Stem Cells in Coculture.Tissue engineering. Part A · 2016Article
- Transitions from mono- to co- to tri-culture uniquely affect gene expression in breast cancer, stromal, and immune compartments.Biomedical microdevices · 2016Article
- Progress towards understanding heterotypic interactions in multi-culture models of breast cancer.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Fibronectin Deposition Participates in Extracellular Matrix Assembly and Vascular Morphogenesis.PloS one · 2016Article
- Vascular morphogenesis of human umbilical vein endothelial cells on cell-derived macromolecular matrix microenvironment.Tissue engineering. Part A · 2014Article
- Hypoxia and the extracellular matrix: drivers of tumour metastasis.Nature reviews. Cancer · 2014Review
- Decorin deficiency promotes hepatic carcinogenesis.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Decorin interferes with platelet-derived growth factor receptor signaling in experimental hepatocarcinogenesis.The FEBS journal · 2013Article
- Breast tumor and stromal cell responses to TGF-β and hypoxia in matrix deposition.Matrix biology : journal of the International Society for Matrix Biology · 2013Review
- Hypoxia Affects the Structure of Breast Cancer Cell-Derived Matrix to Support Angiogenic Responses of Endothelial Cells.Journal of carcinogenesis & mutagenesis · 2013Article
- Localization of decorin gene expression in normal human breast tissue and in benign and malignant tumors of the human breast.Histochemistry and cell biology · 2013Article
- A novel in vitro model for microvasculature reveals regulation of circumferential ECM organization by curvature.PloS one · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The extracellular matrix (ECM), important for maintaining tissue homeostasis, is abnormally expressed in mammary tumors and additionally plays a crucial role in angiogenesis. We hypothesize that breast cancer cells (BCCs) deposit ECM that supports unique patterns of vascular morphogenesis of endothelial cells (ECs). Evaluation of ECM expression revealed that a nontumorigenic cell line (MCF10A), a tumorigenic cell line (MCF7), and a metastatic cell line (MDA-MB-231) express collagens I and IV, fibronectin, and laminin, with tenascin-C limited to MCF10A and MCF7. The amount of ECM deposited by BCCs was found to be higher in MCF10A compared with MCF7 and MDA231, with all ECM differing in their gross structure but similar in mean fiber diameter. Nonetheless, deposition of ECM from BCC lines was overall difficult to detect and insufficient to support capillary-like structure (CLS) formation of ECs. Therefore, a coculture approach was undertaken in which individual BCC lines were cocultured with fibroblasts. Variation in abundance of deposited ECM, deposition of ECM proteins, such as absent collagen I deposition from MDA231-fibroblast cocultures, and fibril organization was found. Deposited ECM from fibroblasts and each coculture supported rapid CLS formation of ECs. Evaluation of capillary properties revealed that CLS grown on ECM deposited from MDA231-fibroblast cocultures possessed significantly larger lumen diameters, occupied the greatest percentage of area, expressed the highest levels of von Willebrand factor, and expressed the greatest amount of E-selectin, which was upregulated independent of exposure to TNF-α. To our knowledge, this is the first study to report tumor cell ECM-mediated differences in vascular capillary features, and thus offers the framework for future investigations interrogating the role of the tumor ECM in supporting vascular morphogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.