ArticlePloS one2012
Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- The role of nucleoside triphosphate hydrolase metallochaperones in making metalloenzymes.Metallomics : integrated biometal science · 2022Review
- Bimodal Nickel-Binding Site onInorganic chemistry · 2019Article
- Nickel Metalloregulators and Chaperones.Inorganics · 2019Article
- Switch I-dependent allosteric signaling in a G-protein chaperone-BThe Journal of biological chemistry · 2017Article
- A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1.PloS one · 2017Article
- Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase.Foodborne pathogens and disease · 2016Article
- Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- RF3:GTP promotes rapid dissociation of the class 1 termination factor.RNA (New York, N.Y.) · 2014Article
- Relationship between Ni(II) and Zn(II) coordination and nucleotide binding by the Helicobacter pylori [NiFe]-hydrogenase and urease maturation factor HypB.The Journal of biological chemistry · 2014Article
- Crystal structures of the carbamoylated and cyanated forms of HypE for [NiFe] hydrogenase maturation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Autoinhibition and signaling by the switch II motif in the G-protein chaperone of a radical B12 enzyme.The Journal of biological chemistry · 2013Article
- Structure of UreG/UreF/UreH complex reveals how urease accessory proteins facilitate maturation of Helicobacter pylori urease.PLoS biology · 2013Article
- Specific metal recognition in nickel trafficking.Biochemistry · 2012Review
- Exploring potassium-dependent GTP hydrolysis in TEES family GTPases.FEBS open bio · 2012Article
- Interaction between hydrogenase maturation factors HypA and HypB is required for [NiFe]-hydrogenase maturation.PloS one · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maturation of [NiFe]-hydrogenase requires the insertion of iron, cyanide and carbon monoxide, followed by nickel, to the catalytic core of the enzyme. Hydrogenase maturation factor HypB is a metal-binding GTPase that is essential for the nickel delivery to the hydrogenase. Here we report the crystal structure of Archeoglobus fulgidus HypB (AfHypB) in apo-form. We showed that AfHypB recognizes guanine nucleotide using Asp-194 on the G5 loop despite having a non-canonical NKxA G4-motif. Structural comparison with the GTPγS-bound Methanocaldococcus jannaschii HypB identifies conformational changes in the switch I region, which bring an invariant Asp-72 to form an intermolecular salt-bridge with another invariant residue Lys-148 upon GTP binding. Substitution of K148A abolished GTP-dependent dimerization of AfHypB, but had no significant effect on the guanine nucleotide binding and on the intrinsic GTPase activity. In vivo complementation study in Escherichia coli showed that the invariant lysine residue is required for in vivo maturation of hydrogenase. Taken together, our results suggest that GTP-dependent dimerization of HypB is essential for hydrogenase maturation. It is likely that a nickel ion is loaded to an extra metal binding site at the dimeric interface of GTP-bound HypB and transferred to the hydrogenase upon GTP hydrolysis.
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