ArticleInternational journal of nanomedicine2012
Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering.
Article in International journal of nanomedicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Histochemistry for Molecular Imaging in Nanomedicine.International journal of molecular sciences · 2024Review
- Multi-Modal Optical Imaging and Combined Phototherapy of Nasopharyngeal Carcinoma Based on a Nanoplatform.International journal of nanomedicine · 2022Review
- Evaluation of 'green' synthesis and biological activity of gold nanoparticles usingIET nanobiotechnology · 2018Article
- Surface enhanced Raman scattering (SERS) based biomicrofluidics systems for trace protein analysis.Biomicrofluidics · 2018Review
- Role of Raman spectroscopy and surface enhanced Raman spectroscopy in colorectal cancer.World journal of gastrointestinal oncology · 2016Review
- Preliminary study of differentiating smears from cancerous and non-cancerous nasopharyngeal tissue using confocal Raman spectroscopy.Journal of cancer research and clinical oncology · 2016Article
- Epstein-Barr Virus-associated lymphoproliferative disorders: experimental and clinical developments.International journal of clinical and experimental medicine · 2015Review
- Recent developments in multiplexing techniques for immunohistochemistry.Expert review of molecular diagnostics · 2015Review
- Surface-enhanced Raman spectroscopy of saliva proteins for the noninvasive differentiation of benign and malignant breast tumors.International journal of nanomedicine · 2015Article
- Actively targeted in vivo multiplex detection of intrinsic cancer biomarkers using biocompatible SERS nanotags.Scientific reports · 2014Article
- Label-free quantitative mass spectrometry from formalin-fixed paraffin-embedded samples of nasopharyngeal carcinoma: Preliminary results from a non-endemic European cohort of patients.Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation OncologyArticle
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10 authors.
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Abstract
backgroundPrevious studies have shown that Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is closely associated with the occurrence and development of nasopharyngeal carcinoma, and can be used as a tumor marker in screening for the disease. Here we report a new methodology based on highly specific and sensitive surface-enhanced Raman scattering (SERS) technology to detect LMP1 in nasopharyngeal tissue sections directly with no need of tedious procedures as with conventional immunohistochemistry methods.
methodsLMP1-functionalized 4-mercaptobenzoic acid (4-MBA)-labeled Au/Ag core-shell bimetallic nanoparticles were prepared first and then applied for analyzing LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections obtained from 34 cancer patients and 20 healthy controls. SERS spectra were acquired from a 25 × 25 spot square area on each tissue section and used to generate SERS images.
resultsData from SERS spectra and images show that this new SERS-based immunoassay detected LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections with high sensitivity and specificity. The results from the new LMP1-SERS probe method are superior to those of conventional immunohistochemistry staining for LMP1, and in excellent agreement with those of in situ hybridization for EBV-encoded small RNA (EBER).
conclusionThis new SERS technique has the potential to be developed into a new clinical tool for detection and differential diagnosis of nasopharyngeal carcinoma as well as for predicting metastasis and immune-targeted treatment of nasopharyngeal carcinoma.
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