ArticleDevelopment (Cambridge, England)2012
Programmed reduction of ABC transporter activity in sea urchin germline progenitors.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Live imaging of echinoderm embryos to illuminate evo-devo.Frontiers in cell and developmental biology · 2022Article
- Early patterning of ABCB, ABCC, and ABCG transporters establishes unique territories of small molecule transport in embryonic mesoderm and endoderm.Developmental biology · 2021Article
- Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Evidence that ABC transporter-mediated autocrine export of an eicosanoid signaling molecule enhances germ cell chemotaxis in the colonial tunicateDevelopment (Cambridge, England) · 2020Article
- Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).Methods in cell biology · 2019Review
- A vesicular Na+/Ca2+ exchanger in coral calcifying cells.PloS one · 2018Article
- Functional diversification of sea urchin ABCC1 (MRP1) by alternative splicing.American journal of physiology. Cell physiology · 2016Article
- Germ Line Versus Soma in the Transition from Egg to Embryo.Current topics in developmental biology · 2015Review
- Transport in technicolor: mapping ATP-binding cassette transporters in sea urchin embryos.Molecular reproduction and development · 2014Review
- Deadenylase depletion protects inherited mRNAs in primordial germ cells.Development (Cambridge, England) · 2014Article
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- Migration of sea urchin primordial germ cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- A high throughput flow cytometric assay platform targeting transporter inhibition.Drug discovery today. Technologies · 2014Review
- Origin and development of the germ line in sea stars.Genesis (New York, N.Y. : 2000) · 2014Review
- Piwi regulates Vasa accumulation during embryogenesis in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Multidrug-resistant transport activity protects oocytes from chemotherapeutic agents and changes during oocyte maturation.Fertility and sterility · 2013Article
- ABC transporters in Dictyostelium discoideum development.PloS one · 2013Article
- Localization and substrate selectivity of sea urchin multidrug (MDR) efflux transporters.The Journal of biological chemistry · 2012Article
- Actin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvilli.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
ATP-binding cassette (ABC) transporters protect embryos and stem cells from mutagens and pump morphogens that control cell fate and migration. In this study, we measured dynamics of ABC transporter activity during formation of sea urchin embryonic cells necessary for the production of gametes, termed the small micromeres. Unexpectedly, we found small micromeres accumulate 2.32 times more of the ABC transporter substrates calcein-AM, CellTrace RedOrange, BoDipy-verapamil and BoDipy-vinblastine, than any other cell in the embryo, indicating a reduction in multidrug efflux activity. The reduction in small micromere ABC transporter activity is mediated by a pulse of endocytosis occurring 20-60 minutes after the appearance of the micromeres--the precursors of the small micromeres. Treating embryos with phenylarsine oxide, an inhibitor of endocytosis, prevents the reduction of transporter activity. Tetramethylrhodamine dextran and cholera toxin B uptake experiments indicate that micromeres have higher rates of bulk and raft-associated membrane endocytosis during the window of transporter downregulation. We hypothesized that this loss of efflux transport could be required for the detection of developmental signaling molecules such as germ cell chemoattractants. Consistent with this hypothesis, we found that the inhibition of ABCB and ABCC-types of efflux transporters disrupts the ordered distribution of small micromeres to the left and right coelomic pouches. These results point to tradeoffs between signaling and the protective functions of the transporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.