ArticlePloS one2012
Isoform diversity and regulation in peripheral and central neurons revealed through RNA-Seq.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 51 citations in OpenAlex.
- c-Jun in neurodegeneration: A key transcriptional regulator with therapeutic implications.Molecular therapy. Nucleic acids · 2026Review
- Endogenous Glycoprotein GPM6a Is Involved in Neurite Outgrowth in Rat Dorsal Root Ganglion Neurons.Biomolecules · 2023Article
- Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.Neuron · 2022Article
- Neuronal Redevelopment and the Regeneration of Neuromodulatory Axons in the Adult Mammalian Central Nervous System.Frontiers in cellular neuroscience · 2022Review
- Phenotypic Screening Following Transcriptomic Deconvolution to Identify Transcription Factors Mediating Axon Growth Induced by a Kinase Inhibitor.SLAS discovery : advancing life sciences R & D · 2021Article
- Birth of a Regulatory Long Non-coding RNA/Gene,Frontiers in genetics · 2021Article
- The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway.The Journal of biological chemistry · 2020Article
- isoTarget: A Genetic Method for Analyzing the Functional Diversity of Splicing Isoforms In Vivo.Cell reports · 2020Article
- Spliced genes in muscle from Nelore Cattle and their association with carcass and meat quality.Scientific reports · 2020Article
- Highly conserved molecular pathways, including Wnt signaling, promote functional recovery from spinal cord injury in lampreys.Scientific reports · 2018Article
- Transcriptome profile of the human placenta.Functional & integrative genomics · 2017Article
- Identification of miRNAs involved in DRG neurite outgrowth and their putative targets.FEBS letters · 2017Article
- Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration.Molecular and cellular neurosciences · 2017Review
- Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Large-scale identification of human cerebrovascular proteins: Inter-tissue and intracerebral vascular protein diversity.PloS one · 2017Article
- Evolution to the rescue: using comparative genomics to understand long non-coding RNAs.Nature reviews. Genetics · 2016Review
- Hyperactivated Stat3 boosts axon regeneration in the CNS.Experimental neurology · 2016Article
- Cell type-specific transcriptome profiling in mammalian brains.Frontiers in bioscience (Landmark edition) · 2016Review
- Neuronal networks in mental diseases and neuropathic pain: Beyond brain derived neurotrophic factor and collapsin response mediator proteins.World journal of psychiatry · 2016Review
- Transcriptome Analysis of the Human Striatum in Tourette Syndrome.Biological psychiatry · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To fully understand cell type identity and function in the nervous system there is a need to understand neuronal gene expression at the level of isoform diversity. Here we applied Next Generation Sequencing of the transcriptome (RNA-Seq) to purified sensory neurons and cerebellar granular neurons (CGNs) grown on an axonal growth permissive substrate. The goal of the analysis was to uncover neuronal type specific isoforms as a prelude to understanding patterns of gene expression underlying their intrinsic growth abilities. Global gene expression patterns were comparable to those found for other cell types, in that a vast majority of genes were expressed at low abundance. Nearly 18% of gene loci produced more than one transcript. More than 8000 isoforms were differentially expressed, either to different degrees in different neuronal types or uniquely expressed in one or the other. Sensory neurons expressed a larger number of genes and gene isoforms than did CGNs. To begin to understand the mechanisms responsible for the differential gene/isoform expression we identified transcription factor binding sites present specifically in the upstream genomic sequences of differentially expressed isoforms, and analyzed the 3' untranslated regions (3' UTRs) for microRNA (miRNA) target sites. Our analysis defines isoform diversity for two neuronal types with diverse axon growth capabilities and begins to elucidate the complex transcriptional landscape in two neuronal populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.