ArticlePloS one2012
AFM-detected apoptotic changes in morphology and biophysical property caused by paclitaxel in Ishikawa and HeLa cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 148 citations in OpenAlex.
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- Exploring the heterogeneity in glioblastoma cellular mechanics using in-vitro assays and atomic force microscopy.Scientific reports · 2025Article
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- Characteristics of Microparticles Based on Resorbable Polyhydroxyalkanoates Loaded with Antibacterial and Cytostatic Drugs.International journal of molecular sciences · 2023Article
- A Response to Article "Selenium-Modified Chitosan Induces HepG2 Cell Apoptosis and Differential Protein Analysis" [Letter].Cancer management and research · 2023Article
- Nanoscale Features of Gambogic Acid Induced ROS-Dependent Apoptosis in Esophageal Cancer Cells Imaged by Atomic Force Microscopy.Scanning · 2022Article
- Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy.Beilstein journal of nanotechnology · 2022Article
- A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide.Chemical science · 2021Article
- Fabricating Silicon Resonators for Analysing Biological Samples.Micromachines · 2021Review
- Biomechanical Properties of Cancer Cells.Cells · 2021Review
- Chemotherapy-Enriched THBS2-Deficient Cancer Stem Cells Drive Hepatocarcinogenesis through Matrix Softness Induced Histone H3 Modifications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- AFM Analysis Enables Differentiation between Apoptosis, Necroptosis, and Ferroptosis in Murine Cancer Cells.iScience · 2020Article
- Furan-Conjugated Tripeptides as Potent Antitumor Drugs.Biomolecules · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The apoptosis of cancer cells is associated with changes in the important cell properties including morphology, surface roughness and stiffness. Therefore, the changes in morphology and biophysical properties can be a good way of evaluating the anticancer activity of a drug. This study examined the effect of paclitaxel on the properties of Ishikawa and HeLa cells using atomic force microscopy (AFM), and the relationship between the changes in morphology and the biophysical properties and apoptosis was discussed. The viability and proliferation of the cells were analyzed using the methylthiazol tetrazolium (MTT) method and a TUNEL assay to confirm cellular apoptosis due to a paclitaxel treatment. AFM observations clearly showed the apoptotic morphological and biophysical changes in Ishikawa and HeLa cells. After the paclitaxel treatment, the cell membrane was torn and holed, the surface roughness was increased, and the stiffness was decreased. These changes were observed more apparently after a 24 h treatment and in Ishikawa cells compared to HeLa cells. The MTT and TUNEL assays results revealed the Ishikawa cells to be more sensitive to paclitaxel than HeLa cells and definite apoptosis occurred after a 24 h treatment. These results showed good agreement with the AFM results. Therefore, research on the morphological and biophysical changes by AFM in cancer cells will help to evaluate the anticancer activities of the drugs.
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