ArticlePloS one2012
Continuous and long-term volume measurements with a commercial Coulter counter.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
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- TORC1 and PKA activity towards ribosome biogenesis oscillates in synchrony with the budding yeast cell cycle.Journal of cell science · 2022Article
- Measuring the average cell size and width of its distribution in cellular tissues using Fourier transform.The European physical journal. E, Soft matter · 2022Article
- Microfluidic Single-Cell Analytics.Advances in biochemical engineering/biotechnology · 2022Article
- A single Na+-Pi cotransporter in Toxoplasma plays key roles in phosphate import and control of parasite osmoregulation.PLoS pathogens · 2020Article
- Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast.Nature cell biology · 2019Article
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- Cell Swelling Induced by the Antimalarial KAE609 (Cipargamin) and Other PfATP4-Associated Antimalarials.Antimicrobial agents and chemotherapy · 2018Article
- Effect of Temperature on the Size Distribution, Shell Properties, and Stability of DefinityUltrasound in medicine & biology · 2018Article
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Functional Mitochondria Are Important for the Effect of Resveratrol.Molecules (Basel, Switzerland) · 2017Article
- Large population cell characterization using quantitative phase cytometer.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
- Osmotic behaviour of human mesenchymal stem cells: Implications for cryopreservation.PloS one · 2017Article
- Optical volume and mass measurements show that mammalian cells swell during mitosis.The Journal of cell biology · 2015Article
- Microfluidic impedance flow cytometry enabling high-throughput single-cell electrical property characterization.International journal of molecular sciences · 2015Review
- Translate to divide: сontrol of the cell cycle by protein synthesis.Microbial cell (Graz, Austria) · 2015Review
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- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Live-cell mass profiling: an emerging approach in quantitative biophysics.Nature methods · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate a method to enhance the time resolution of a commercial Coulter counter and enable continuous and long-term cell size measurements for growth rate analyses essential to understanding basic cellular processes, such as cell size regulation and cell cycle progression. Our simple modifications to a commercial Coulter counter create controllable cell culture conditions within the sample compartment and combine temperature control with necessary adaptations to achieve measurement stability over several hours. We also wrote custom software, detailed here, to analyze instrument data files collected by either this continuous method or standard, periodic sampling. We use the continuous method to measure the growth rate of yeast in G1 during a prolonged arrest and, in different samples, the dependency of growth rate on cell size and cell cycle position in arrested and proliferating cells. We also quantify with high time resolution the response of mouse lymphoblast cell culture to drug treatment. This method provides a technique for continuous measurement of cell size that is applicable to a large variety of cell types and greatly expands the set of analysis tools available for the Coulter counter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.