ArticlePloS one2012
A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Accurate and sensitive TaqMan real-time PCR assays to detect and quantify simian malaria parasites Plasmodium cynomolgi, Plasmodium inui and Plasmodium coatneyi.Malaria journal · 2026Article
- BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay TargetingPathogens (Basel, Switzerland) · 2024Article
- Article
- Development of two multiplex PCR assays for rapid detection of eleven Gram-negative bacteria in children with septicemia.Tropical medicine and health · 2024Article
- Virtual Multiplexing Chamber-Based Digital PCR for Camel Milk Authentication Applications.Micromachines · 2023Article
- Nucleic Acid Detection with Ion Concentration Polarization Microfluidic Chip for Reduced Cycle Numbers of Polymerase Chain Reaction.Micromachines · 2022Article
- Development and application of DNA markers to detect adulteration withFood science and biotechnology · 2022Article
- Review
- A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator.Scientific reports · 2019Article
- GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target Genomes.Frontiers in microbiology · 2019Article
- A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses.BMC veterinary research · 2018Article
- Analysis of swine leukocyte antigen class I gene profiles and porcine endogenous retrovirus viremia level in a transgenic porcine herd inbred for xenotransplantation research.Journal of veterinary science · 2018Article
- Article
- A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples.International journal of molecular sciences · 2016Article
- Development and application of loop-mediated isothermal amplification assays for rapid visual detection of cry2Ab and cry3A genes in genetically-modified crops.International journal of molecular sciences · 2014Article
- A novel universal multiplex PCR improves detection of AZFc Y-chromosome microdeletions.Journal of assisted reproduction and genetics · 2014Article
- Randomly broken fragment PCR with 5' end-directed adaptor for genome walking.Scientific reports · 2013Article
- Automatic analysis of 2D polyacrylamide gels in the diagnosis of DNA polymorphisms.Biomedical engineering online · 2013Article
- A novel multiplex PCR for detection of Pseudomonas aeruginosa: A major cause of wound infections.Pakistan journal of medical sciences · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5'-end of each specific primer pairs which matched with the specific DNA sequences for each template and also used as the universal primer (UP). PCR products were analyzed on sequencing gel electrophoresis (SGE) which had the advantage of exhibiting extraordinary resolution. This method overcame the disadvantages rooted deeply in conventional multiplex PCR such as complex manipulation, lower sensitivity, self-inhibition and amplification disparity resulting from different primers, and it got a high specificity and had a low detection limit of 0.1 ng for single kind of crops when screening the presence of genetically modified (GM) crops in mixture samples. The novel developed multiplex PCR assay with sequencing gel electrophoresis analysis will be useful in many fields, such as verifying the GM status of a sample irrespective of the crop and GM trait and so on.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.