ReviewActa biochimica et biophysica Sinica2012
A primer on using pooled shRNA libraries for functional genomic screens.
Review in Acta biochimica et biophysica Sinica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- 8-Aminoguanine protects against paclitaxel-induced neural degeneration and mechanical allodynia.JCI insight · 2026Article
- Functional Genomics: Principles and Methods.Advances in experimental medicine and biology · 2026Review
- In Vivo Genome-Wide Pooled RNAi Screens in Cancer Cells to Identify Determinants of Chemotherapy/Drug Response.Methods in molecular biology (Clifton, N.J.) · 2021Article
- MasterPATH: network analysis of functional genomics screening data.BMC genomics · 2020Article
- Decoding pluripotency: Genetic screens to interrogate the acquisition, maintenance, and exit of pluripotency.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Forward genetic screen in human podocytes identifies diphthamide biosynthesis genes as regulators of adhesion.American journal of physiology. Renal physiology · 2019Article
- Beta-binomial modeling of CRISPR pooled screen data identifies target genes with greater sensitivity and fewer false negatives.Genome research · 2019Article
- Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm.PloS one · 2018Article
- Intron-specific shRNA-mediated downregulation of survivin and promotion of apoptosis in HeLa cells.Oncology letters · 2017Article
- Sources of Error in Mammalian Genetic Screens.G3 (Bethesda, Md.) · 2016Article
- A synthetic-lethality RNAi screen reveals an ERK-mTOR co-targeting pro-apoptotic switch in PIK3CA+ oral cancers.Oncotarget · 2016Article
- Patient Mutation Directed shRNA Screen Uncovers Novel Bladder Tumor Growth Suppressors.Molecular cancer research : MCR · 2015Article
- Toward stem cell-based phenotypic screens for neurodegenerative diseases.Nature reviews. Neurology · 2015Review
- Genome-wide shRNA screening to identify factors mediating Gata6 repression in mouse embryonic stem cells.Development (Cambridge, England) · 2013Article
- Functional genomic screening to enhance oncolytic virotherapy.British journal of cancer · 2013Review
- Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.PloS one · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The discovery of RNA interference (RNAi) has revolutionized genetic analysis in mammalian cells. Loss-of-function RNAi screens enable rapid, functional annotation of the genome. Of the various RNAi approaches, pooled shRNA libraries have received considerable attention because of their versatility. A number of genome-wide shRNA libraries have been constructed against the human and mouse genomes, and these libraries can be readily applied to a variety of screens to interrogate the function of human and mouse genes in an unbiased fashion. We provide an introduction to the technical aspects of using pooled shRNA libraries for genetic screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.