ArticleIn vitro cellular & developmental biology. Animal2012
Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line.
Article in In vitro cellular & developmental biology. Animal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Novel Insight into the Role of Squalene Epoxidase (International journal of molecular sciences · 2023Article
- Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection.Cell communication and signaling : CCS · 2023Article
- G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway.Cell cycle (Georgetown, Tex.) · 2022Article
- Inhibition of cell proliferation and promotion of acinus-like structure formation from goat mammary epithelial cells via Wnt/β-catenin signaling.In vitro cellular & developmental biology. Animal · 2021Article
- Article
- Effect of incubation temperature on lactogenic function of goat milk-derived mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2020Article
- Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions.Cytotechnology · 2018Article
- Regulation of peroxisome proliferator-activated receptor gamma on milk fat synthesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2016Article
- Biological characterization of bovine mammary epithelial cell lines immortalized by HPV16 E6/E7 and SV40T.In vitro cellular & developmental biology. Animal · 2016Article
- Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?Biological research · 2016Article
- 14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2015Article
- Lineage-specific markers of goat mammary cells in primary culture.In vitro cellular & developmental biology. Animal · 2014Article
- Establishment and characterization of dairy cow growth hormone secreting anterior pituitary cell model.In vitro cellular & developmental biology. Animal · 2014Article
- Establishment of mammary gland model in vitro: culture and evaluation of a yak mammary epithelial cell line.PloS one · 2014Article
- Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line.In vitro cellular & developmental biology. Animal · 2013Article
- Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study milk synthesis in dairy goat mammary gland, we had established an in vitro lactating dairy goat mammary epithelial cell (DGMEC) line. Mammary tissues of Guan Zhong dairy goats at 35 d of lactation were dispersed and cultured in a medium containing epithelial growth factor, insulin-like growth factor-1, insulin transferrin serum, and fetal bovine serum. Epithelial cells were enriched by digesting with 0.25% trypsin repeatedly to remove fibroblast cells and were identified as epithelial origin by staining with antibody against cytokeratine 18. The DGMECs displayed monolayer, cobble-stone, epithelial-like morphology, and formed alveoli-like structures and island monolayer aggregates which were the typical characteristics of mammary epithelial cells. A one-half logarithmically growth curve and cytoplasmic lipid droplets in these cells were observed. In this paper, we also studied the lactating function of DGMECs. Results showed that DGMECs could secrete lactose and β-casein. Lactating function of the cells had no obvious change after 48 h treated by insulin, while prolactin could obviously raise the secretion of milk proteins and lactose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.