ArticleJournal of industrial microbiology & biotechnology2012
Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds.Frontiers in plant science · 2023Article
- Activity-Based Screening of Soil Samples from Nyingchi, Tibet, for Amylase-Producing Bacteria and Other Multifunctional Enzyme Capacities.International journal of microbiology · 2022Article
- Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern.Frontiers in microbiology · 2022Article
- Biochemical and Structural Characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family.Computational and structural biotechnology journal · 2021Article
- Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan.Microbial cell factories · 2019Article
- Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library.Frontiers in microbiology · 2019Article
- Est10: A Novel Alkaline Esterase Isolated from Bovine Rumen Belonging to the New Family XV of Lipolytic Enzymes.PloS one · 2015Article
- Characterization of EstB, a novel cold-active and organic solvent-tolerant esterase from marine microorganism Alcanivorax dieselolei B-5(T).Extremophiles : life under extreme conditions · 2014Article
- New extremophilic lipases and esterases from metagenomics.Current protein & peptide science · 2014Review
- Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea brine pool.Scientific reports · 2013Article
- Isolation and characterization of a metagenome-derived thermoalkaliphilic esterase with high stability over a broad pH range.Extremophiles : life under extreme conditions · 2013Article
- Characterization of three new carboxylic ester hydrolases isolated by functional screening of a forest soil metagenomic library.Journal of industrial microbiology & biotechnology · 2013Article
- Characterization and application of a newly synthesized 2-deoxyribose-5-phosphate aldolase.Journal of industrial microbiology & biotechnology · 2013Article
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7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and the enzyme was characterized. The estIM1 gene had an open reading frame (ORF) of 936 base pairs and encoded a protein of 311 amino acids with a molecular mass 34 kDa and a pI value of 4.32. The deduced amino acid sequence was 62% identical to that of an esterase from an uncultured bacterium (ABQ11271). The amino acid sequence indicated that EstIM1 was a member of the family IV of lipolytic enzymes, all of which contain a GDSAG motif shared with similar enzymes of lactic acid microorganisms. EstIM1 was active over a temperature range of 1-50°C, at alkaline pH. The activation energy for hydrolysis of p-nitrophenyl propionate was 1.04 kcal/mol, within a temperature range of 1-40°C. The activity of EstIM1 was about 60% of maximal even at 1°C, suggesting that EstIM1 is efficiently cold-adapted. Further characterization of this cold-adapted enzyme indicated that the esterase may be very valuable in industrial applications.
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