ArticlePlanta2012
Characterization of recombinant Beta vulgaris 4,5-DOPA-extradiol-dioxygenase active in the biosynthesis of betalains.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 90 citations in OpenAlex.
- A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa.The New phytologist · 2026Article
- A functional betalain-producing dioxygenase inIMA fungus · 2026Article
- Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli.Nature communications · 2025Article
- Identification ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Structural Insights into 4,5-DOPA Extradiol Dioxygenase fromJournal of agricultural and food chemistry · 2025Article
- In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase.PloS one · 2025Article
- High-efficiency production of plant-derived pigment dopaxanthin in Escherichia coli by combination engineering.Microbial cell factories · 2024Article
- Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.BMC plant biology · 2023Article
- Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor.BMC plant biology · 2023Article
- Regulation Mechanism of Plant Pigments Biosynthesis: Anthocyanins, Carotenoids, and Betalains.Metabolites · 2022Review
- Reannotation of Fly Amanita l-DOPA Dioxygenase Gene Enables Its Cloning and Heterologous Expression.ACS omega · 2022Article
- Transcriptome analyses shed light on floral organ morphogenesis and bract color formation in Bougainvillea.BMC plant biology · 2022Article
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- A chromosome-scale genome sequence of pitaya (Hylocereus undatus) provides novel insights into the genome evolution and regulation of betalain biosynthesis.Horticulture research · 2021Article
- A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (International journal of molecular sciences · 2021Article
- First description of betalains biosynthesis in an aquatic organism: characterization of 4,5-DOPA-extradiol-dioxygenase activity in the cyanobacteria Anabaena cylindrica.Microbial biotechnology · 2020Article
- Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales.The New phytologist · 2020Article
- Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells.Nucleic acids research · 2020Article
- Oxidative opening of the aromatic ring: Tracing the natural history of a large superfamily of dioxygenase domains and their relatives.The Journal of biological chemistry · 2019Article
- Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells.Plant biotechnology journal · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Betalains are water-soluble pigments with high antiradical capacity which bestow bright colors to flowers, fruits and other parts of most plants of the order Caryophyllales. The formation of the structural unit of all betalains, betalamic acid from the precursor amino acid 4,5-dihydroxyphenylalanine is catalyzed by the enzyme 4,5-DOPA-extradiol-dioxygenase followed by intramolecular cyclization of the 4,5-secodopa intermediate. This paper describes the purification and the molecular and functional characterization of an active 4,5-DOPA-extradiol-dioxygenase from the best-known source of betalains-Beta vulgaris-after heterologous expression in Escherichia coli. The enzyme is a monomeric protein with a molecular mass of 32 kDa characterized by chromatography, electrophoresis and MALDI-TOF analysis. Enzyme kinetic properties are characterized in the production of betalamic acid, the structural, chromophoric and bioactive unit of plant pigment betalains.
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