ArticlePlanta2012
ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Defective pollen meiosis in Arabidopsis due to combined arabinan and galactan insufficiency.Plant & cell physiology · 2025Article
- Exogenous auxin-induced rapid modulation of herbaceous peony stem straightness: insights into transient cell wall metabolism responses.Plant cell reports · 2025Article
- Multiprotein Complexes of Plant Glycosyltransferases Involved in Their Function and Trafficking.Plants (Basel, Switzerland) · 2025Review
- Identification of glycosyltransferases mediating 2-O-arabinopyranosyl and 2-O-galactosyl substitutions of glucuronosyl side chains of xylan.The Plant journal : for cell and molecular biology · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- NKS1/ELMO4 is an integral protein of a pectin synthesis protein complex and maintains Golgi morphology and cell adhesion inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genetic dissection of monosaccharides contents in rice whole grain using genome-wide association study.The plant genome · 2023Article
- The cotton pectin methyl esterase gene GhPME21 functions in microspore development and fertility in Gossypium hirsutum L.Plant molecular biology · 2023Article
- Temporal cell wall changes during cold acclimation and deacclimation and their potential involvement in freezing tolerance and growth.Physiologia plantarum · 2023Article
- Altering arabinans increases Arabidopsis guard cell flexibility and stomatal opening.Current biology : CB · 2022Article
- The Modification of Plant Cell Wall Polysaccharides in Potato Plants duringPlants (Basel, Switzerland) · 2021Article
- Natural population re-sequencing detects the genetic basis of local adaptation to low temperature in a woody plant.Plant molecular biology · 2021Article
- A Pipeline towards the Biochemical Characterization of theInternational journal of molecular sciences · 2021Article
- Arabinogalactan Proteins in Plant Roots - An Update on Possible Functions.Frontiers in plant science · 2021Review
- Polysaccharide Biosynthesis: Glycosyltransferases and Their Complexes.Frontiers in plant science · 2021Article
- Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles.Cellular and molecular life sciences : CMLS · 2020Review
- Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106.Frontiers in plant science · 2020Article
- Molecular characteristics of plant UDP-arabinopyranose mutases.Glycobiology · 2019Review
- Separating Golgi Proteins fromThe Plant cell · 2019Article
- Poplar carbohydrate-active enzymes: whole-genome annotation and functional analyses based on RNA expression data.The Plant journal : for cell and molecular biology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosyltransferase complexes are known to be involved in plant cell wall biosynthesis, as for example in cellulose. It is not known to what extent such complexes are involved in biosynthesis of pectin as well. To address this question, work was initiated on ARAD1 (ARABINAN DEFICIENT 1) and its close homolog ARAD2 of glycosyltransferase family GT47. Using bimolecular fluorescence complementation, Förster resonance energy transfer and non-reducing gel electrophoresis, we show that ARAD1 and ARAD2 are localized in the same Golgi compartment and form homo-and heterodimeric intermolecular dimers when expressed transiently in Nicotiana benthamiana. Biochemical analysis of arad2 cell wall or fractions hereof showed no difference in the monosaccharide composition, when compared with wild type. The double mutant arad1 arad2 had an arad1 cell wall phenotype and overexpression of ARAD2 did not complement the arad1 phenotype, indicating that ARAD1 and ARAD2 are not redundant enzymes. To investigate the cell wall structure of the mutants in detail, immunohistochemical analyses were carried out on arad1, arad2 and arad1 arad2 using the arabinan-specific monoclonal antibody LM13. In roots, the labeling pattern of arad2 was distinct from both that of wild type, arad1 and arad1 arad2. Likewise, in epidermal cell walls of inflorescence stems, LM13 binding differed between arad2 and WILD TYPE, arad1 or arad1 arad2. Altogether, these data show that ARAD2 is associated with arabinan biosynthesis, not redundant with ARAD1, and that the two glycosyltransferases may function in complexes held together by disulfide bridges.
Indexed as
Identifiers
22270560What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.