ArticleHistochemistry and cell biology2012
Expression and subcellular distribution of gephyrin in non-neuronal tissues and cells.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Association and functional study ofWorld journal of psychiatry · 2025Article
- Differential Expressions of circRNAs and Regulatory Mechanisms of ceRNA Network in Liver of Wilson's Disease TX Mice.Journal of inflammation research · 2024Article
- Automated Image Analysis Reveals Different Localization of Synaptic Gephyrin C4 Splice Variants.eNeuro · 2023Article
- Article
- GPER1 Modulates Synaptic Plasticity During the Development of Temporal Lobe Epilepsy in Rats.Neurochemical research · 2021Article
- In focus in HCB.Histochemistry and cell biology · 2021Article
- Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270.Histochemistry and cell biology · 2021Article
- Tuning GABAergic Inhibition: Gephyrin Molecular Organization and Functions.Neuroscience · 2020Review
- Gephyrin suppresses lung squamous cell carcinoma development by reducing mTOR pathway activation.Cancer management and research · 2019Article
- Gephyrin: a key regulatory protein of inhibitory synapses and beyond.Histochemistry and cell biology · 2018Review
- KCC2 knockdown impairs glycinergic synapse maturation in cultured spinal cord neurons.Histochemistry and cell biology · 2016Article
- Human gephyrin is encompassed within giant functional noncoding yin-yang sequences.Nature communications · 2015Article
- Gephyrin: a master regulator of neuronal function?Nature reviews. Neuroscience · 2014Review
- Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- Bacterial over-expression and purification of the 3'phosphoadenosine 5'phosphosulfate (PAPS) reductase domain of human FAD synthase: functional characterization and homology modeling.International journal of molecular sciences · 2012Article
- Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin.The Journal of biological chemistry · 2012Article
- Splice-specific glycine receptor binding, folding, and phosphorylation of the scaffolding protein gephyrin.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gephyrin is a scaffolding protein required for the accumulation of inhibitory neurotransmitter receptors at neuronal postsynaptic membranes. In non-neuronal tissues, gephyrin is indispensible for the biosynthesis of molybdenum cofactor, the prosthetic group of oxidoreductases including sulfite oxidase and xanthine oxidase. However, the molecular and cellular basis of gephyrin's non-neuronal function is poorly understood; in particular, the roles of its splice variants remain enigmatic. Here, we used cDNA screening as well as Northern and immunoblot analyses to show that mammalian liver contains only a limited number of gephyrin splice variants, with the C3-containing variant being the predominant isoform. Using new and established anti-gephyrin antibodies in immunofluorescence and subcellular fractionation studies, we report that gephyrin localizes to the cytoplasm of both tissue hepatocytes and cultured immortalized cells. These findings were corroborated by RNA interference studies in which the cytosolic distribution was found to be abolished. Finally, by blue-native PAGE we show that cytoplasmic gephyrin is part of a ~600 kDa protein complex of yet unknown composition. Our data suggest that the expression pattern of non-neuronal gephyrin is simpler than indicated by previous evidence. In addition, gephyrin's presence in a cytosolic 600 kDa protein complex suggests that its metabolic and/or other non-neuronal functions are exerted in the cytoplasm and are not confined to a particular subcellular compartment.
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