ArticleThe Biochemical journal2012
A novel lectin from Agrocybe aegerita shows high binding selectivity for terminal N-acetylglucosamine.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Mushrooms as potent autophagy modulators in cancer therapy: Current evidence and therapeutic prospects.Cancer pathogenesis and therapy · 2026Review
- Systematic Evaluation of Affinity Enrichment Methods for O-GlcNAc Proteomics.Journal of proteome research · 2024Article
- Review
- Article
- Article
- Comprehensive analysis of lectin-glycan interactions reveals determinants of lectin specificity.PLoS computational biology · 2021Article
- A New Lectin fromBioMed research international · 2021Article
- Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer.Glycoconjugate journal · 2019Article
- AANL (Agrocybe aegerita lectin 2) is a new facile tool to probe for O-GlcNAcylation.Glycobiology · 2018Article
- Structural and Functional Characterization of PA14/Flo5-Like Adhesins FromFrontiers in microbiology · 2018Article
- Tongue Sole CD209: A Pattern-Recognition Receptor that Binds a Broad Range of Microbes and Promotes Phagocytosis.International journal of molecular sciences · 2017Article
- DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum.Frontiers in immunology · 2017Article
- Review
- Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.British journal of cancer · 2016Article
- N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression.Molecular & cellular proteomics : MCP · 2016Article
- Nutritional value, chemical composition, antioxidant activity and enrichment of cream cheese with chestnut mushroom Agrocybe aegerita (Brig.) Sing.Journal of food science and technology · 2015Article
- Mushroom lectins: specificity, structure and bioactivity relevant to human disease.International journal of molecular sciences · 2015Review
- Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin.PloS one · 2015Article
- Article
- Proteome exploration to provide a resource for the investigation of Ganoderma lucidum.PloS one · 2015Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel lectin was isolated from the mushroom Agrocybe aegerita (designated AAL-2) by affinity chromatography with GlcNAc (N-acetylglucosamine)-coupled Sepharose 6B after ammonium sulfate precipitation. The AAL-2 coding sequence (1224 bp) was identified by performing a homologous search of the five tryptic peptides identified by MS against the translated transcriptome of A. aegerita. The molecular mass of AAL-2 was calculated to be 43.175 kDa from MS, which was consistent with the data calculated from the amino acid sequence. To analyse the carbohydrate-binding properties of AAL-2, a glycan array composed of 465 glycan candidates was employed, and the result showed that AAL-2 bound with high selectivity to terminal non-reducing GlcNAc residues, and further analysis revealed that AAL-2 bound to terminal non-reducing GlcNAc residues with higher affinity than previously well-known GlcNAc-binding lectins such as WGA (wheatgerm agglutinin) and GSL-II (Griffonia simplicifolia lectin-II). ITC (isothermal titration calorimetry) showed further that GlcNAc bound to AAL-2 in a sequential manner with moderate affinity. In the present study, we also evaluated the anti-tumour activity of AAL-2. The results showed that AAL-2 could bind to the surface of hepatoma cells, leading to induced cell apoptosis in vitro. Furthermore, AAL-2 exerted an anti-hepatoma effect via inhibition of tumour growth and prolongation of survival time of tumour-bearing mice in vivo.
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