ArticleThe Journal of biological chemistry2012
Biochemical characterization of ribosome assembly GTPase RbgA in Bacillus subtilis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L.Nucleic acids research · 2026Article
- The late stages of yeast mitoribosome large subunit biogenesis.Biochimica et biophysica acta. Molecular cell research · 2025Article
- YsxC is a placeholder for ribosomal protein uL2 during 50S ribosomal subunit assembly.Nucleic acids research · 2025Article
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- Article
- Role of GTPases in Driving Mitoribosome Assembly.Trends in cell biology · 2021Review
- Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit.The EMBO journal · 2021Article
- Structural consequences of the interaction of RbgA with a 50S ribosomal subunit assembly intermediate.Nucleic acids research · 2019Article
- The (p)ppGpp-binding GTPase Era promotes rRNA processing and cold adaptation in Staphylococcus aureus.PLoS genetics · 2019Article
- Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.The Journal of biological chemistry · 2018Article
- Article
- MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation.Nucleic acids research · 2018Article
- Functional characterization of chloroplast-targeted RbgA GTPase in higher plants.Plant molecular biology · 2017Article
- Structural and biochemical analysis ofThe Journal of biological chemistry · 2017Article
- Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis.The EMBO journal · 2017Article
- YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit.Nucleic acids research · 2016Article
- ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The K⁺-dependent GTPase Nug1 is implicated in the association of the helicase Dbp10 to the immature peptidyl transferase centre during ribosome maturation.Nucleic acids research · 2016Article
- Histidine 114 Is Critical for ATP Hydrolysis by the Universally Conserved ATPase YchF.The Journal of biological chemistry · 2015Article
- Mitochondrial ribosome assembly in health and disease.Cell cycle (Georgetown, Tex.) · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ribosome biogenesis GTPase A protein RbgA is involved in the assembly of the large ribosomal subunit in Bacillus subtilis, and homologs of RbgA are implicated in the biogenesis of mitochondrial, chloroplast, and cytoplasmic ribosomes in archaea and eukaryotes. The precise function of how RbgA contributes to ribosome assembly is not understood. Defects in RbgA give rise to a large ribosomal subunit that is immature and migrates at 45 S in sucrose density gradients. Here, we report a detailed biochemical analysis of RbgA and its interaction with the ribosome. We found that RbgA, like most other GTPases, exhibits a very slow k(cat) (14 h(-1)) and has a high K(m) (90 μM). Homology modeling of the RbgA switch I region using the K-loop GTPase MnmE as a template suggested that RbgA requires K(+) ions for GTPase activity, which was confirmed experimentally. Interaction with 50 S subunits, but not 45 S intermediates, increased GTPase activity by ∼55-fold. Stable association with 50 S subunits and 45 S intermediates was nucleotide-dependent, and GDP did not support strong interaction with either of the subunits. GTP and guanosine 5'-(β,γ-imido)triphosphate (GMPPNP) were sufficient to promote association with the 45 S intermediate, whereas only GMPPNP was able to support binding to the 50 S subunit, presumably due to the stimulation of GTP hydrolysis. These results support a model in which RbgA promotes a late step in ribosome biogenesis and that one role of GTP hydrolysis is to stimulate dissociation of RbgA from the ribosome.
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Registered trials
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