ArticleApplied and environmental microbiology2012
Behavior and target site selection of conjugative transposon Tn916 in two different strains of toxigenic Clostridium difficile.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
- Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermentingSynthetic and systems biotechnology · 2026Article
- From colonization to infection: Genomic evolution of Clostridioides difficile pathogenesis.Cell reports · 2026Review
- Mobilome of Environmental Isolates ofAntibiotics (Basel, Switzerland) · 2025Article
- Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome.PloS one · 2025Article
- Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.PLoS genetics · 2024Article
- A nisin-inducible chromosomal gene expression system based on ICE Tn5253 of Streptococcus pneumoniae, transferable among streptococci and enterococci.World journal of microbiology & biotechnology · 2024Article
- Integrative and Conjugative Elements and Prophage DNA as Carriers of Resistance Genes inInternational journal of molecular sciences · 2024Article
- Antibiotic Resistances of Clostridioides difficile.Advances in experimental medicine and biology · 2024Article
- Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria.PLoS genetics · 2022Article
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies.PLoS genetics · 2022Article
- Article
- Clostridioides difficile phage biology and application.FEMS microbiology reviews · 2021Review
- Article
- Determination of copy number and circularization ratio of TnJournal of oral microbiology · 2019Article
- Mobile Genetic Elements Associated with Antimicrobial Resistance.Clinical microbiology reviews · 2018Review
- Article
- Autonomous Replication of the Conjugative Transposon Tn916.Journal of bacteriology · 2016Article
- CodY-Dependent Regulation of Sporulation in Clostridium difficile.Journal of bacteriology · 2016Article
- Recent advances in the understanding of antibiotic resistance in Clostridium difficile infection.Therapeutic advances in infectious disease · 2016Review
- Mobile genetic elements in Clostridium difficile and their role in genome function.Research in microbiology · 2015Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
The insertion sites of the conjugative transposon Tn916 in the anaerobic pathogen Clostridium difficile were determined using Illumina Solexa high-throughput DNA sequencing of Tn916 insertion libraries in two different clinical isolates: 630ΔE, an erythromycin-sensitive derivative of 630 (ribotype 012), and the ribotype 027 isolate R20291, which was responsible for a severe outbreak of C. difficile disease. A consensus 15-bp Tn916 insertion sequence was identified which was similar in both strains, although an extended consensus sequence was observed in R20291. A search of the C. difficile 630 genome showed that the Tn916 insertion motif was present 100,987 times, with approximately 63,000 of these motifs located in genes and 35,000 in intergenic regions. To test the usefulness of Tn916 as a mutagen, a functional screen allowed the isolation of a mutant. This mutant contained Tn916 inserted into a gene involved in flagellar biosynthesis.
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