ArticleApplied and environmental microbiology2012
Association with an ammonium-excreting bacterium allows diazotrophic culture of oil-rich eukaryotic microalgae.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 97 citations in OpenAlex.
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Characterising complex metabolic responses in an engineered, cross-feeding microbial co-culture using quantitative proteomics.New biotechnology · 2026Article
- Article
- Increased Apigenin in DNA-Edited Hexaploid Wheat Promoted Soil Bacterial Nitrogen Fixation and Improved Grain Yield Under Limiting Nitrogen Fertiliser.Plant biotechnology journal · 2025Article
- Bioplastic production in air-lift bioreactors using a spent cooking oil-based method for sustainable recovery.World journal of microbiology & biotechnology · 2025Article
- Co-Culture of Cyanobacteria and Rhizobia, Increasing Biomass Under Nitrogen-Starvation Conditions.Applied biochemistry and biotechnology · 2025Article
- Azotobacter vinelandii as a Nitrogen-Negative Chassis for Bio-Oil and Bio-Wax Production of Heterologous and Native Lipids.MicrobiologyOpen · 2025Article
- Enhanced Urea Production in the Diazotroph Azotobacter vinelandii as a Means of Stable Nitrogen Biofertiliser Production.Microbial biotechnology · 2025Article
- Applying LFQRatio Normalization in Quantitative Proteomic Analysis of Microbial Co-culture Systems.Bio-protocol · 2025Article
- Engineering nitrogen-fixing microbiomes with waste-derived carbon sources: toward circular and resilient biofertilizer solution.Frontiers in microbiology · 2025Article
- Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance inFrontiers in microbiology · 2025Article
- Article
- A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae andBio-protocol · 2024Article
- Precision control of ammonium release in Azotobacter vinelandii.Microbial biotechnology · 2024Article
- Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.Applied microbiology and biotechnology · 2024Article
- LFQRatio: A Normalization Method to Decipher Quantitative Proteome Changes in Microbial Coculture Systems.Journal of proteome research · 2024Article
- Enhanced extracellular ammonium release in the plant endophyteMicrobiology spectrum · 2024Article
- Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.ACS synthetic biology · 2023Article
- Microalgal and Nitrogen-Fixing Bacterial Consortia: From Interaction to Biotechnological Potential.Plants (Basel, Switzerland) · 2023Review
- Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-FixingMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Concerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third-generation biofuels from microalgal biomass mainly because of its potential for high yields and reduced land use changes in comparison with biofuels derived from plant feedstocks. Regardless of the nature of the feedstock, the use of fertilizers, especially nitrogen, entails a potential economic and environmental drawback for the sustainability of biofuel production. In this work, we have studied the possibility of nitrogen biofertilization by diazotrophic bacteria applied to cultured microalgae as a promising feedstock for next-generation biofuels. We have obtained an Azotobacter vinelandii mutant strain that accumulates several times more ammonium in culture medium than wild-type cells. The ammonium excreted by the mutant cells is bioavailable to promote the growth of nondiazotrophic microalgae. Moreover, this synthetic symbiosis was able to produce an oil-rich microalgal biomass using both carbon and nitrogen from the air. This work provides a proof of concept that artificial symbiosis may be considered an alternative strategy for the low-N-intensive cultivation of microalgae for the sustainable production of next-generation biofuels and other bioproducts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.