ArticleMolecular & cellular proteomics : MCP2012
Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Linking kinetochore attachment to checkpoint control: the role of Aurora B in BubR1 acetylation.Nucleic acids research · 2026Article
- Ubiquitin specific protease 7 deubiquitinates and regulates Aurora B-mediated cytokinesis.BMB reports · 2025Article
- Review
- An Uncommon Phosphorylation Mode Regulates the Activity and Protein Interactions ofJournal of the American Chemical Society · 2024Article
- Phosphoproteomic Characterization and Kinase Signature Predict Response to Venetoclax Plus 3+7 Chemotherapy in Acute Myeloid Leukemia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Interaction of PINK1 with nucleotides and kinetin.Science advances · 2024Article
- A non-catalytic N-terminus domain of WRN prevents mitotic telomere deprotection.Scientific reports · 2023Article
- The ubiquitin-ligase TRAF6 and TGFβ type I receptor form a complex with Aurora kinase B contributing to mitotic progression and cytokinesis in cancer cells.EBioMedicine · 2022Article
- Molecular Epigenetics: Chemical Biology Tools Come of Age.Annual review of biochemistry · 2021Review
- An analog-sensitive allele of Aurora kinase B is lethal in mouse.microPublication biology · 2021Article
- Phospho-regulation of mitotic spindle assembly.Cytoskeleton (Hoboken, N.J.) · 2020Review
- Cell division requires RNA eviction from condensing chromosomes.The Journal of cell biology · 2020Article
- PLK1 plays dual roles in centralspindlin regulation during cytokinesis.The Journal of cell biology · 2019Article
- Late mitotic functions of Aurora kinases.Chromosoma · 2017Review
- Plant Aurora kinases interact with and phosphorylate transcription factors.Journal of plant research · 2016Article
- Article
- A novel protein kinase is essential in bloodstream Trypanosoma brucei.International journal for parasitology · 2016Article
- CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis.Current biology : CB · 2016Article
- HP1-Assisted Aurora B Kinase Activity Prevents Chromosome Segregation Errors.Developmental cell · 2016Article
- Identification of AMPK Phosphorylation Sites Reveals a Network of Proteins Involved in Cell Invasion and Facilitates Large-Scale Substrate Prediction.Cell metabolism · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
To understand how the chromosomal passenger complex ensures chromosomal stability, it is crucial to identify its substrates and to find ways to specifically inhibit the enzymatic core of the complex, Aurora B. We therefore developed a chemical genetic approach to selectively inhibit human Aurora B. By mutating the gatekeeper residue Leu-154 in the kinase active site, the ATP-binding pocket was enlarged, but kinase function was severely disrupted. A unique second site suppressor mutation was identified that rescued kinase activity in the Leu-154 mutant and allowed the accommodation of bulky N(6)-substituted adenine analogs. Using this analog-sensitive Aurora B kinase, we found that retention of the chromosomal passenger complex at the centromere depends on Aurora B kinase activity. Furthermore, analog-sensitive Aurora B was able to use bulky ATPγS analogs and could thiophosphorylate multiple proteins in cell extracts. Utilizing an unbiased approach for kinase substrate mapping, we identified several novel substrates of Aurora B, including the nucleosomal-binding protein HMGN2. We confirmed that HMGN2 is a bona fide Aurora B substrate in vivo and show that its dynamic association to chromatin is controlled by Aurora B.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.