ArticleAquatic toxicology (Amsterdam, Netherlands)2012
Functional characterization of four metallothionein genes in Daphnia pulex exposed to environmental stressors.
Article in Aquatic toxicology (Amsterdam, Netherlands), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Metallothionein and neurodegenerative diseases.Neural regeneration research · 2026Article
- Discovery and Evaluation of Cadmium-Adapted Daphnia pulex Genotypes in a Region of Historical Mining Reveals Adaptation Protects the Germline From Cadmium-Induced Mutations.Molecular ecology · 2026Article
- Myriapod Metallothioneins Conserve the Ancestral Architecture of Arthropods While Displaying Lineage-Specific Adaptations in Metal Binding.Genome biology and evolution · 2026Article
- History of exposure to copper influences transgenerational gene expression responses inEpigenetics · 2024Article
- Article
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas.Environmental science and pollution research international · 2020Article
- Fitness and Genomic Consequences of Chronic Exposure to Low Levels of Copper and Nickel inG3 (Bethesda, Md.) · 2019Article
- Phase 0 of the Xenobiotic Response: Nuclear Receptors and Other Transcription Factors as a First Step in Protection from Xenobiotics.Nuclear receptor research · 2019Article
- Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.PloS one · 2015Article
- Expression of metallothionein of freshwater crab (Sinopotamon henanense) in Escherichia coli enhances tolerance and accumulation of zinc, copper and cadmium.Ecotoxicology (London, England) · 2014Article
- Transcription patterns of genes encoding four metallothionein homologs in Daphnia pulex exposed to copper and cadmium are time- and homolog-dependent.Aquatic toxicology (Amsterdam, Netherlands) · 2013Article
- A pharm-ecological perspective of terrestrial and aquatic plant-herbivore interactions.Journal of chemical ecology · 2013Review
- Molecular cloning and expression of novel metallothionein (MT) gene in the polychaete Perinereis nuntia exposed to metals.Environmental science and pollution research international · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We characterized the metallothionein genes (Mt1, Mt2, Mt3, and Mt4) in Daphnia pulex on both molecular and ecotoxicological level. We therefore conducted a bioinformatical analysis of the gene location and predicted protein sequence, and screened the upstream flanking region for regulatory elements. The number of these elements and their positions relative to the start codon varied strongly among the four genes and even among two gene duplicates (Mt1A and Mt1B), suggesting different roles of the four proteins in the organisms' response to stress. We subsequently conducted a chronic 16-day exposure of D. pulex to different environmental stressors (at sublethal levels causing approximately 50% reduction in reproduction). Based on prior knowledge, we exposed them to the metals Cd, Cu, and Ni, the moulting hormone hydroxyecdysone (20E), and the oxidative stressors cyanobacteria (Microcystis aeruginosa), and paraquat (Pq). We then compared mRNA expression levels of the four Mt genes under these stress conditions with control conditions in "The Chosen One" clone (TCO), for which the full genome was sequenced and annotated. All together, the mRNA expression results under the different stress regimes indicate that different Mt genes may play different and various roles in the response of D. pulex to stress and that some (but not all) of the differences among the four genes could be related to the pattern of regulatory elements in their upstream flanking region.
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