ArticleNature methods2012
Chronic in vivo imaging in the mouse spinal cord using an implanted chamber.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
What it found
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Who cites it
100 citing papers in PubMed.
- Microglial Labeling in Mouse Spinal Cord Injury: A Review of Timing, Efficacy, and Specificity.Glia · 2026Review
- Myelin sheaths persist for weeks following axon degeneration.Science advances · 2026Article
- Differential modification of ascending spinal outputs in acute and chronic pain states.Neuron · 2025Article
- Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy.Cancer immunology, immunotherapy : CII · 2025Review
- Spike rate inference from mouse spinal cord calcium imaging data.bioRxiv : the preprint server for biology · 2025Article
- Long-term optical imaging of the spinal cord in awake behaving mice.Nature methods · 2024Article
- A biomimic anti-neuroinflammatory nanoplatform for active neutrophil extracellular traps targeting and spinal cord injury therapy.Materials today. Bio · 2024Article
- Imaging Approaches in Cancer Biology.Cold Spring Harbor perspectives in medicine · 2024Review
- Exploring the role of spinal astrocytes in the onset of hyperalgesic priming signals in acid-induced chronic muscle pain.PNAS nexus · 2024Article
- In Vivo Imaging of Rabbit Follicles through Combining Ultrasound Bio-Microscopy and Intravital Window.Animals : an open access journal from MDPI · 2024Article
- Ultraflexible electrodes for recording neural activity in the mouse spinal cord during motor behavior.Cell reports · 2024Article
- Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cordbioRxiv : the preprint server for biology · 2024Article
- Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain.Neurophotonics · 2024Article
- Review
- 3D-printed weight holders design and testing in mouse models of spinal cord injury.Frontiers in drug delivery · 2024Article
- Sensory Processing of Cutaneous Temperature in the Peripheral and Central Nervous System.Advances in experimental medicine and biology · 2024Review
- Towards a Brighter Constellation: Multi-Organ Neuroimaging of Neural and Vascular Dynamics in the Spinal Cord and Brain.bioRxiv : the preprint server for biology · 2023Article
- Anatomical Diversity of the Adult Corticospinal Tract Revealed by Single-Cell Transcriptional Profiling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Pathological hemodynamic changes and leukocyte transmigration disrupt the blood-spinal cord barrier after spinal cord injury.Journal of neuroinflammation · 2023Article
- Evaluation of a gain-managed nonlinear fiber amplifier for multiphoton microscopy.Biomedical optics express · 2023Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Understanding and treatment of spinal cord pathology is limited in part by a lack of time-lapse in vivo imaging strategies at the cellular level. We developed a chronically implanted spinal chamber and surgical procedure suitable for time-lapse in vivo multiphoton microscopy of mouse spinal cord without the need for repeat surgical procedures. We routinely imaged mice repeatedly for more than 5 weeks postoperatively with up to ten separate imaging sessions and observed neither motor-function deficit nor neuropathology in the spinal cord as a result of chamber implantation. Using this chamber we quantified microglia and afferent axon dynamics after a laser-induced spinal cord lesion and observed massive microglia infiltration within 1 d along with a heterogeneous dieback of axon stumps. By enabling chronic imaging studies over timescales ranging from minutes to months, our method offers an ideal platform for understanding cellular dynamics in response to injury and therapeutic interventions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.