ArticleCarcinogenesis2012
Generation of reactive oxygen species by grape seed extract causes irreparable DNA damage leading to G2/M arrest and apoptosis selectively in head and neck squamous cell carcinoma cells.
Article in Carcinogenesis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- α- and β-Casein Differentially Protect Anthocyanins from Three Red Grape (Foods (Basel, Switzerland) · 2026Article
- Grape seed extract and L-ascorbic acid exert antineoplastic effects against solid Ehrlich carcinomaFrontiers in immunology · 2025Article
- Effects of Ethanol Concentrations on Primary Structural and Bioactive Characteristics ofNutrients · 2024Article
- Antioxidant and Anticancer Activity ofLife (Basel, Switzerland) · 2024Article
- The association between the Mediterranean Diet Score and death from cancer of the head and neck.Cancer causes & control : CCC · 2024Article
- Grape Seed Components as Protectors of Inflammation, DNA Damage, and Cancer.Current nutrition reports · 2023Review
- Metabolomic Profiling and Assessment of Phenolic Compounds Derived from Vitis davidii Foex Cane and Stem ExtractsInternational journal of molecular sciences · 2022Article
- Article
- Grape seed procyanidin extract against lung cancer: the role of microrna-106b, bioavailability, and bioactivity.Oncotarget · 2018Article
- Grape Seed Procyanidin Extract Mediates Antineoplastic Effects against Lung Cancer via Modulations of Prostacyclin and 15-HETE Eicosanoid Pathways.Cancer prevention research (Philadelphia, Pa.) · 2016Article
- Cardioprotective Effect of Grape Seed Extract on Chronic Doxorubicin-Induced Cardiac Toxicity in Wistar Rats.Advanced pharmaceutical bulletin · 2016Article
- MicroRNA-19a/b mediates grape seed procyanidin extract-induced anti-neoplastic effects against lung cancer.The Journal of nutritional biochemistry · 2016Article
- Oxidative stress in oral diseases.Oral diseases · 2016Review
- Grape seed extract targets mitochondrial electron transport chain complex III and induces oxidative and metabolic stress leading to cytoprotective autophagy and apoptotic death in human head and neck cancer cells.Molecular carcinogenesis · 2015Article
- Grape seed extract and resveratrol prevent 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by modulating AMPK activation and associated biological responses.Molecular carcinogenesis · 2015Article
- Concentration effects of grape seed extracts in anti-oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage.BMC complementary and alternative medicine · 2015Article
- Pachymic acid induces apoptosis via activating ROS-dependent JNK and ER stress pathways in lung cancer cells.Cancer cell international · 2015Article
- Implications of the colonic deposition of free hemoglobin-α chain: a previously unknown tissue by-product in inflammatory bowel disease.Inflammatory bowel diseases · 2014Article
- Role of oxidative stress in cytotoxicity of grape seed extract in human bladder cancer cells.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2013Article
- Bitter melon juice activates cellular energy sensor AMP-activated protein kinase causing apoptotic death of human pancreatic carcinoma cells.Carcinogenesis · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Head and neck squamous cell carcinoma (HNSCC) accounts for 6% of all malignancies in USA and unfortunately the recurrence of secondary primary tumors and resistance against conventional treatments decrease the overall 5 year survival rate in HNSCC patients. Thus, additional approaches are needed to control HNSCC. Here, for the first time, employing human HNSCC Detroit 562 and FaDu cells as well as normal human epidermal keratinocytes, we investigate grape seed extract (GSE) efficacy and associated mechanism in both cell culture and nude mice xenografts. GSE selectively inhibited the growth and caused cell cycle arrest and apoptotic death in both Detroit 562 and FaDu cells by activating DNA damage checkpoint cascade, including ataxia telangiectasia mutated/ataxia telangiectasia-Rad3-related-checkpoint kinase 1/2-cell division cycle 25C as well as caspases 8, 9 and 3. Consistent with these results, GSE treatment resulted in a strong DNA damage and a decrease in the levels of DNA repair molecules breast cancer gene 1 and Rad51 and DNA repair foci. GSE-caused accumulation of intracellular reactive oxygen species was identified as a major mechanism of its effect for growth inhibition, DNA damage and apoptosis, which was remarkably reversed by antioxidant N-acetylcysteine. GSE feeding to nude mice decreased Detroit 562 and FaDu xenograft tumor growth by 67 and 65% (P < 0.001), respectively. In immunohistochemical analysis, xenografts from GSE-fed groups showed decreased proliferation but increased DNA damage and apoptosis. Together, these findings show that GSE targets both DNA damage and repair and provide mechanistic insights for its efficacy selectively against HNSCC both in cell culture and mouse xenograft, supporting its translational potential against HNSCC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.