ArticleFEBS letters2012
Single-particle tracking of immunoglobulin E receptors (FcεRI) in micron-sized clusters and receptor patches.
Article in FEBS letters, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- RBL-2H3 Mast Cell Receptor Dynamics in the Immunological Synapse.Biophysica · 2022Article
- Review
- CPB-3 and CGH-1 localize to motile particles within dendrites in C. elegans PVD sensory neurons.BMC research notes · 2021Article
- DARC, Glycophorin A, Band 3, and GLUT1 Diffusion in Erythrocytes: Insights into Membrane Complexes.Biophysical journal · 2020Article
- Article
- Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.Journal of imaging · 2019Article
- A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor.Methods and protocols · 2019Review
- From Dynamics to Membrane Organization: Experimental Breakthroughs Occasion a "Modeling Manifesto".Biophysical journal · 2018Review
- Toward Hydrodynamics with Solvent Free Lipid Models: STRD Martini.Biophysical journal · 2016Article
- TIRF imaging of Fc gamma receptor microclusters dynamics and signaling on macrophages during frustrated phagocytosis.BMC immunology · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- N-terminal amphipathic helix of Amphiphysin can change the spatial distribution of immunoglobulin E receptors (FcεRI) in the RBL-2H3 mast cell synapse.Results in immunology · 2016Article
- Dances with Membranes: Breakthroughs from Super-resolution Imaging.Current topics in membranes · 2015Review
- Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy.Nucleic acids research · 2014Article
- Article
- Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Distinct stages of stimulated FcεRI receptor clustering and immobilization are identified through superresolution imaging.Biophysical journal · 2013Article
- Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
When mast cells contact a monovalent antigen-bearing fluid lipid bilayer, IgE-loaded FcεRI receptors aggregate at contact points and trigger degranulation and the release of immune activators. We used two-color total internal reflection fluorescence microscopy and single-particle tracking to show that most fluorescently labeled receptor complexes diffuse freely within these micron-size clusters, with a diffusion coefficient comparable to free receptors in resting cells. At later times, when the small clusters coalesce to form larger patches, receptors diffuse even more rapidly. In all cases, Monte Carlo diffusion simulations ensured that the tracking results were free of bias, and distinguished biological from statistical variation. These results show the diversity in receptor mobility in mast cells, demonstrating at least three distinct states of receptor diffusivity.
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Registered trials
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