ReviewMethods in enzymology2012
Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy.
Review in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 75 citations in OpenAlex.
- Bound or unbound: mapping and monitoring receptor oligomerization by time-resolved fluorescence live-cell imaging.Frontiers in molecular biosciences · 2026Article
- Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.Scientific reports · 2025Article
- Potent Fluorescent Probe for Target-Engagement Studies of Allosteric Pyruvate Kinase Modulators.Angewandte Chemie (International ed. in English) · 2025Article
- Emerging ultrafast technologies in biotechnology.3 Biotech · 2025Review
- Enzymatic Synthesis of Functional PEGylated Adipate Copolymers.ChemPlusChem · 2025Article
- Applications of Artificial Intelligence, Deep Learning, and Machine Learning to Support the Analysis of Microscopic Images of Cells and Tissues.Journal of imaging · 2025Review
- Label-free metabolic imaging ofFrontiers in cellular and infection microbiology · 2025Article
- Article
- Interferometric excitation fluorescence lifetime imaging microscopy.Nature communications · 2024Article
- Implementation of FRET Spectrometry Using Temporally Resolved Fluorescence: A Feasibility Study.International journal of molecular sciences · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.Biophysical reports · 2023Article
- Article
- Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues.Frontiers in immunology · 2023Article
- Article
- High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts.ACS photonics · 2022Article
- Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics.Methods in enzymology · 2020Article
- FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope.Journal of biomedical optics · 2019Article
- Fast fit-free analysis of fluorescence lifetime imaging via deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET.Bio-protocol · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside single living cells, such as monitoring changes in intracellular ions and detecting protein-protein interactions. Here, we describe the digital frequency domain FLIM data acquisition and analysis. We describe the methods necessary to calibrate the FLIM system and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from Förster Resonance Energy Transfer (FRET). We show how the "FRET-standard" fusion proteins are used to validate the FLIM system for FRET measurements. We then show how FLIM-FRET can be used to detect the dimerization of the basic leucine zipper (B Zip) domain of the transcription factor CCAAT/enhancer binding protein α in the nuclei of living mouse pituitary cells. Importantly, the factors required for the accurate determination and reproducibility of lifetime measurements are described in detail.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.