ArticleThe Journal of biological chemistry2012
Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor.Molecules (Basel, Switzerland) · 2024Article
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- In Silico Identification and Biological Evaluation of Antioxidant Food Components Endowed with IX and XIIAntioxidants (Basel, Switzerland) · 2020Article
- The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis.International journal of molecular sciences · 2020Review
- New 8-Molecules (Basel, Switzerland) · 2020Article
- Tendril extract ofInternational journal of medical sciences · 2020Article
- Expression and functional analyses of a putative phenylcoumaran benzylic ether reductase in Arabidopsis thaliana.Plant cell reports · 2016Article
- 18O-Tracer Metabolomics Reveals Protein Turnover and CDP-Choline Cycle Activity in Differentiating 3T3-L1 Pre-Adipocytes.PloS one · 2016Article
- Effective suppression of pro-inflammatory molecules by DHCA via IKK-NF-κB pathway, in vitro and in vivo.British journal of pharmacology · 2015Article
- Berberine Suppresses Adipocyte Differentiation via Decreasing CREB Transcriptional Activity.PloS one · 2015Article
- Plant natural products as an anti-lipid droplets accumulation agent.Journal of natural medicines · 2014Review
- Pumpkin (Cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice.Molecules (Basel, Switzerland) · 2012Article
- Efficacy of Sweet Pumpkin in Relieving Contact Dermatitis in Chronically Stressed Rats.Journal of microscopy and ultrastructureArticle
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A water-soluble extract from the stems of Cucurbita moschata, code named PG105, was previously found to contain strong anti-obesity activities in a high fat diet-induced obesity mouse model. One of its biological characteristics is that it inhibits 3T3-L1 adipocyte differentiation. To isolate the biologically active compound(s), conventional solvent fractionation was performed, and the various fractions were tested for anti-adipogenic activity using Oil Red O staining method. A single spot on thin layer chromatography of the chloroform fraction showed a potent anti-adipogenic activity. When purified, the structure of its major component was resolved as dehydrodiconiferyl alcohol (DHCA), a lignan, by NMR and mass spectrometry analysis. In 3T3-L1 cells, synthesized DHCA significantly reduced the expression of several adipocyte marker genes, including peroxisome proliferator-activated receptor γ (Pparg), CCAAT/enhancer-binding protein α (Cebpa), fatty acid-binding protein 4 (Fabp4), sterol response element-binding protein-1c (Srebp1c), and stearoyl-coenzyme A desaturase-1 (Scd), and decreased lipid accumulation without affecting cell viability. DHCA also suppressed the mitotic clonal expansion of preadipocytes (an early event of adipogenesis), probably by suppressing the DNA binding activity of C/EBPβ, and lowered the production level of cyclinA and cyclin-dependent kinase 2 (Cdk2), coinciding with the decrease in DNA synthesis and cell division. In addition, DHCA directly inhibited the expression of SREBP-1c and SCD-1. Similar observations were made, using primary mouse embryonic fibroblasts. Taken together, our data indicate that DHCA may contain dual activities, affecting both adipogenesis and lipogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.