ArticleJournal of lipid research2012
Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.Journal of the American Society for Mass Spectrometry · 2024Review
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Soft Matrix-Assisted Laser Desorption/Ionization for Labile Glycoconjugates.Journal of the American Society for Mass Spectrometry · 2019Article
- Age-dependent and regional heterogeneity in the long-chain base of A-series gangliosides observed in the rat brain using MALDI Imaging.Scientific reports · 2017Article
- "Magic" Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Drug detection and quantification directly from tissue using novel ionization methods for mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2015Article
- Article
- Transmission geometry laserspray ionization vacuum using an atmospheric pressure inlet.Analytical chemistry · 2014Article
- Structural characterization of gangliosides and glycolipids via ultraviolet photodissociation mass spectrometry.Analytical chemistry · 2013Article
- High-speed tandem mass spectrometric in situ imaging by nanospray desorption electrospray ionization mass spectrometry.Analytical chemistry · 2013Article
- Qualitative and quantitative mass spectrometry imaging of drugs and metabolites.Advanced drug delivery reviews · 2013Review
- Matrix assisted ionization vacuum (MAIV), a new ionization method for biological materials analysis using mass spectrometry.Molecular & cellular proteomics : MCP · 2013Article
- A mechanism for ionization of nonvolatile compounds in mass spectrometry: considerations from MALDI and inlet ionization.Journal of the American Society for Mass Spectrometry · 2012Article
- Matrix assisted ionization: new aromatic and nonaromatic matrix compounds producing multiply charged lipid, peptide, and protein ions in the positive and negative mode observed directly from surfaces.Journal of the American Society for Mass Spectrometry · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A new ionization method for the analysis of fragile gangliosides without undesired fragmentation or salt adduction is presented. In laserspray ionization inlet (LSII), the matrix/analyte sample is ablated at atmospheric pressure, and ionization takes place in the ion transfer capillary of the mass spectrometer inlet by a process that is independent of a laser wavelength or voltage. The softness of LSII allows the identification of gangliosides up to GQ1 with negligible sialic acid loss. This is of importance to the field of MS imaging, as undesired fragmentation has made it difficult to accurately map the spatial distribution of fragile ganglioside lipids in tissue. Proof-of-principle structural characterization of endogenous gangliosides using MS(n) fragmentation of multiply charged negative ions on a LTQ Velos and subsequent imaging of the GD1 ganglioside is demonstrated. This is the first report of multiply charged negative ions using inlet ionization. We find that GD1 is detected at higher levels in the mouse cortex and hippocampus compared with the thalamus. In LSII with the laser aligned in transmission geometry relative to the inlet, images were obtained in approximately 60 min using an inexpensive nitrogen laser.
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Registered trials
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