Evidence map›Paper›PMID 22262367›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012

Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples.

P Goñi, B Martín, M Villacampa, A García, C Seral, F J Castillo, A Clavel

Abstract readComparative StudyEvaluation Study
PubMed Publisher
In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 76 citations in OpenAlex.

  1. Article
  2. Analysis of Reported Cases ofMicroorganisms · 2025
    Article
  3. Journal of clinical medicine · 2024
    Review
  4. Diagnosis and control of cryptosporidiosis in farm animals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022
    Review
  5. Accurate laboratory diagnosis of human intestinal and extra-intestinal amoebiasis.Gastroenterology and hepatology from bed to bench · 2022
    Review
  6. Article
  7. Food and waterborne parasitology · 2021
    Review
  8. Article
  9. Article
  10. Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020
    Article
  11. Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans.Frontiers in cellular and infection microbiology · 2020
    Review
  12. Update on laboratory diagnosis of amoebiasis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019
    Review
  13. Giardia lamblia infection: review of current diagnostic strategies.Gastroenterology and hepatology from bed to bench · 2019
    Review
  14. Article
  15. Article
  16. Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016
    Article
  17. Article
  18. Detection of Entamoeba histolytica by Recombinase Polymerase Amplification.The American journal of tropical medicine and hygiene · 2015
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

P GoñiArea of Parasitology, Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009, Zaragoza, Spain.
B Martín
M Villacampa
A García
C Seral
F J Castillo
A Clavel
Universidad de Zaragoza · ESHospital Clínico Universitario Lozano Blesa · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunochromatographic (IC) tests may play an important role in the future diagnosis of parasitic diseases because of their speed and simplicity of use. A recently developed test to detect Cryptosporidium spp, Giardia duodenalis and Entamoeba histolytica was evaluated. Microscopy and PCR were the "gold standard" reference techniques and the results of this IC test were compared with those obtained with ELISA and IC single test for the three parasites. One hundred sixty stool samples were assayed. Using microscopy, 22 samples were diagnosed as positive for Cryptosporidium spp., 31 for Giardia duodenalis, 41 for Entamoeba histolytica/dispar, and 68 had a negative diagnosis for the three parasites. Results of IC tests show sensitivities of 70-72% for Cryptosporidium, 90-97% for Giardia and 62.5% for Entamoeba histolytica. Specificities were of 93.6-94.9%, >99% and 96.1%, respectively. In all diagnoses, agreement with microscopy and PCR was over 90%, except in the triple test and microscopy in E. histolytica detection that was 76.3%, due to the inability of microscopy to differentiate E. histolytica from nonpathogenic species such as E. dispar or E. moshkovskii. The triple stool immunoassays provide adequate sensitivities and specificities for use in outbreak situations, for screening proposals and for massive assays in endemic areas where a large number of samples must be analysed or as complementary test for individual diagnosis.

Indexed as

Antigens, ProtozoanChromatography, AffinityCryptosporidiosisCryptosporidiumEntamoeba histolyticaEntamoebiasisEnzyme-Linked Immunosorbent AssayFecesGiardia lambliaGiardiasisHumansMicroscopyPolymerase Chain ReactionSensitivity and SpecificityAntigens, Protozoan

Identifiers

PMID22262367
OpenAlexW2066487719

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.