ArticleNature protocols2012
In vitro culture of epicardial cells from adult zebrafish heart on a fibrin matrix.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Sulf1 regulates inflammation via modulation of TGF-β dependent epicardial activation.EMBO reports · 2026Article
- Agarose-Based 3D Invasion Assay for Simultaneous Quantification of Tumor Cell Invasion and Extracellular Matrix Degradation.Methods and protocols · 2026Article
- Macrophages and cardiac lesion in zebrafish: what can single-cell RNA sequencing reveal?Frontiers in cardiovascular medicine · 2025Review
- Hypoxia promotes a perinatal-like progenitor state in the adult murine epicardium.Scientific reports · 2022Article
- Activation of Nkx2.5 transcriptional program is required for adult myocardial repair.Nature communications · 2022Article
- Hydrogels derived from acellular porcine corneal stroma enhance corneal wound healing.Acta biomaterialia · 2021Article
- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device.Lab on a chip · 2020Article
- Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium.Nature materials · 2019Article
- Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart.Development (Cambridge, England) · 2019Article
- Engineering cardiac microphysiological systems to model pathological extracellular matrix remodeling.American journal of physiology. Heart and circulatory physiology · 2018Review
- Tension Creates an Endoreplication Wavefront that Leads Regeneration of Epicardial Tissue.Developmental cell · 2017Article
- Recent advancements in understanding endogenous heart regeneration-insights from adult zebrafish and neonatal mice.Seminars in cell & developmental biology · 2016Review
- Explant culture of adult zebrafish hearts for epicardial regeneration studies.Nature protocols · 2016Article
- Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration.Journal of visualized experiments : JoVE · 2016Article
- Extracellular component hyaluronic acid and its receptor Hmmr are required for epicardial EMT during heart regeneration.Cardiovascular research · 2015Article
- Article
- The Epicardium in the Embryonic and Adult Zebrafish.Journal of developmental biology · 2014Article
- The zebrafish as a model for complex tissue regeneration.Trends in genetics : TIG · 2013Review
- Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts.Nature protocols · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We describe here a protocol for culturing epicardial cells from adult zebrafish hearts, which have a unique regenerative capacity after injury. Briefly, zebrafish hearts first undergo ventricular amputation or sham operation. Next, the hearts are excised and explanted onto fibrin gels prepared in advance in a multiwell tissue culture plate. The procedure allows the epicardial cells to outgrow from the ventricle onto a fibrin matrix in vitro. This protocol differs from those used in other organisms by using a fibrin gel to mimic blood clots that normally form after injury and that are essential for proper cell migration. The culture procedure can be accomplished within 5 h; epicardial cells can be obtained within 24-48 h and can be maintained in culture for 5-6 d. This protocol can be used to investigate the mechanisms underlying epicardial cell migration, proliferation and epithelial-to-mesenchymal transition during heart regeneration, homeostatic cardiac growth or other physiological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.