ArticleHistochemistry and cell biology2012
Cholinergic chemosensory cells in the auditory tube.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Physiological functions and pharmacological targeting of transient receptor potential channels.Pharmacological reviews · 2025Review
- Extragustatory bitter taste receptors in head and neck health and disease.Journal of molecular medicine (Berlin, Germany) · 2024Review
- Article
- Evaluation of eGFP expression in the ChAT-eGFP transgenic mouse brain.BMC neuroscience · 2023Article
- Tuft Cells Are Present in Submandibular Glands Across Species.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2022Article
- Identification of cholinergic cells with chemosensory traits in the porcine uterus.Cell and tissue research · 2022Article
- Novel protocol to observe the intestinal tuft cell using transmission electron microscopy.Biology open · 2022Article
- Isolation, Ex Vivo Culture, and Stimulation of Tracheal and Nasal Chemosensory Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cellular and functional heterogeneity of the airway epithelium.Mucosal immunology · 2021Review
- Development of epithelial cholinergic chemosensory cells of the urethra and trachea of mice.Cell and tissue research · 2021Article
- Tuft and Cancer Stem Cell Marker DCLK1: A New Target to Enhance Anti-Tumor Immunity in the Tumor Microenvironment.Cancers · 2020Review
- Acetylcholine From Tuft Cells: The Updated Insights Beyond Its Immune and Chemosensory Functions.Frontiers in cell and developmental biology · 2020Review
- Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas.Journal of visualized experiments : JoVE · 2019Article
- Tuft Cells-Systemically Dispersed Sensory Epithelia Integrating Immune and Neural Circuitry.Annual review of immunology · 2019Review
- The Immune Function of Tuft Cells at Gut Mucosal Surfaces and Beyond.Journal of immunology (Baltimore, Md. : 1950) · 2019Review
- Detection of intrinsic cholinergic system in the human lacrimal drainage system: evidence and potential implications.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
- Extraoral bitter taste receptors in health and disease.The Journal of general physiology · 2017Review
- Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice.PloS one · 2017Article
- Chemosensory epithelial cells in the urethra: sentinels of the urinary tract.Histochemistry and cell biology · 2016Review
- Co-expression of GAD67 and choline acetyltransferase reveals a novel neuronal phenotype in the mouse medulla oblongata.Autonomic neuroscience : basic & clinical · 2015Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The luminal composition of the auditory tube influences its function. The mechanisms involved in the monitoring are currently not known. For the lower respiratory epithelium, such a sentinel role is carried out by cholinergic brush cells. Here, using two different mouse strains expressing eGFP under the control of the promoter of choline acetyltransferase (ChAT), we show the presence of solitary cholinergic villin-positive brush cells also in the mouse auditory tube epithelium. They express the vesicular acetylcholine (ACh) transporter and proteins of the taste transduction pathway such as α-gustducin, phospholipase C beta 2 (PLC(β2)) and transient receptor potential cation channel subfamily M member 5 (TRPM5). Immunoreactivity for TRPM5 and PLCβ2 was found regularly, whereas α-gustducin was absent in approximately 15% of the brush cells. Messenger RNA for the umami taste receptors (TasR), Tas1R1 and 3, and for the bitter receptors, Tas2R105 and Tas2R108, involved in perception of cycloheximide and denatonium were detected in the auditory tube. Using a transgenic mouse that expresses eGFP under the promotor of the nicotinic ACh receptor α3-subunit, we identified cholinoceptive nerve fibers that establish direct contacts to brush cells in the auditory tube. A subpopulation of these fibers displayed also CGRP immunoreactivity. Collectively, we show for the first time the presence of brush cells in the auditory tube. These cells are equipped with all proteins essential for sensing the composition of the luminal microenvironment and for communication of the changes to the CNS via attached sensory nerve fibers.
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