ArticleBiophysical journal2011
Measuring kinetic dissociation/association constants between Lactococcus lactis bacteria and mucins using living cell probes.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.Advanced drug delivery reviews · 2023Review
- Antioxidant Activities of Aqueous Extracts and Protein Hydrolysates from Marine Worm Hechong (Foods (Basel, Switzerland) · 2022Article
- Interplay of receptor-ligand binding and lipid domain formation during cell adhesion.Frontiers in molecular biosciences · 2022Review
- The biophysics of bacterial infections: Adhesion events in the light of force spectroscopy.Cell surface (Amsterdam, Netherlands) · 2021Article
- Elucidating bacterial adhesion to mucosal surface by an original AFM approach.BMC microbiology · 2021Article
- From the first touch to biofilm establishment by the human pathogen Candida glabrata: a genome-wide to nanoscale view.Communications biology · 2021Article
- Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion.Frontiers in molecular biosciences · 2021Article
- The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?Microorganisms · 2018Review
- A novel protocol to prepare cell probes for the quantification of microbial adhesion and biofilm initiation on structured bioinspired surfaces using AFM for single-cell force spectroscopy: Dedicated to Prof. em. Dr. Dr. H.C. Karl Schügerl on the occasion of his 90th birthday.Engineering in life sciences · 2017Article
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Ligand binding pocket of a novel Allatostatin receptor type C of stick insect, Carausius morosus.Scientific reports · 2017Article
- Surface Proteins ofFrontiers in microbiology · 2017Review
- Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants.Applied microbiology and biotechnology · 2016Article
- Spatial Localization and Binding of the Probiotic Lactobacillus farciminis to the Rat Intestinal Mucosa: Influence of Chronic Stress.PloS one · 2015Article
- Article
- Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy.Nature protocols · 2014Article
- Toward atomic force microscopy and mass spectrometry to visualize and identify lipid rafts in plasmodesmata.Frontiers in plant science · 2014Article
- Single-cell force spectroscopy of probiotic bacteria.Biophysical journal · 2013Article
- Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work we focused on quantifying adhesion between Lactococcus lactis, the model for lactic acid bacteria (LAB) and mucins. Interactions between two strains of L. lactis (IBB477 and MG1820 as control) and pig gastric mucin-based coating were measured and compared with the use of atomic force microscopy. Analysis of retraction force-distance curves shed light on the differential contributions of nonspecific and specific forces. An increased proportion of specific adhesive events was obtained for IBB477 (20% vs. 5% for the control). Blocking assays with free pig gastric mucin and its O-glycan moiety showed that oligosaccharides play a major (but not exclusive) role in L. lactis-mucins interactions. Specific interactions were analyzed in terms of kinetic constants. An increase in the loading rate of atomic force microscope tip led to a higher force between interacting biological entities, which was directly linked to the kinetic dissociation constant (K(off)). Enhancing the contact time between the tip and the sample allowed an increase in the interaction probability, which can be related to the kinetic association constant (K(on)). Variations in the loading rate and contact time enabled us to determine K(on) (3.3 × 10(2) M(-1)·s(-1)) and K(off) (0.46 s(-1)), and the latter was consistent with values given in the literature for sugar-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.