ArticleAMB Express2012
Desiccation induces viable but Non-Culturable cells in Sinorhizobium meliloti 1021.
Article in AMB Express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Post-Inoculation Drying and Storage Effects on HAB Viability and Nutrient Retention in Biochar.Biotech (Basel (Switzerland)) · 2026Article
- The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria.Microorganisms · 2023Review
- Extracellular DNAses Facilitate Antagonism and Coexistence in Bacterial Competitor-Sensing Interference Competition.Applied and environmental microbiology · 2022Article
- Effects of rehydration on physiological and transcriptional responses of a water-stressed rhizobium.Journal of microbiology (Seoul, Korea) · 2022Article
- Characterization of the endophytic bacterial community ofFrontiers in plant science · 2022Article
- Programmable design of seed coating function induces water-stress tolerance in semi-arid regions.Nature food · 2021Article
- A bioinspired approach to engineer seed microenvironment to boost germination and mitigate soil salinity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy.PloS one · 2019Article
- Maintenance and assessment of cell viability in formulation of non-sporulating bacterial inoculants.Microbial biotechnology · 2018Review
- Persistence in Phytopathogenic Bacteria: Do We Know Enough?Frontiers in microbiology · 2018Review
- Xerotolerant bacteria: surviving through a dry spell.Nature reviews. Microbiology · 2017Review
- The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage.Microbial cell factories · 2014Article
- Identification and characterization of a NaCl-responsive genetic locus involved in survival during desiccation in Sinorhizobium meliloti.Applied and environmental microbiology · 2013Article
- Salt tolerance in Astragalus cicer microsymbionts: the role of glycine betaine in osmoprotection.Current microbiology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sinorhizobium meliloti is a microorganism commercially used in the production of e.g. Medicago sativa seed inocula. Many inocula are powder-based and production includes a drying step. Although S. meliloti survives drying well, the quality of the inocula is reduced during this process. In this study we determined survival during desiccation of the commercial strains 102F84 and 102F85 as well as the model strain USDA1021.The survival of S. meliloti 1021 was estimated during nine weeks at 22% relative humidity. We found that after an initial rapid decline of colony forming units, the decline slowed to a steady 10-fold reduction in colony forming units every 22 days. In spite of the reduction in colony forming units, the fraction of the population identified as viable (42-54%) based on the Baclight live/dead stain did not change significantly over time. This change in the ability of viable cells to form colonies shows (i) an underestimation of the survival of rhizobial cells using plating methods, and that (ii) in a part of the population desiccation induces a Viable But Non Culturable (VBNC)-like state, which has not been reported before. Resuscitation attempts did not lead to a higher recovery of colony forming units indicating the VBNC state is stable under the conditions tested. This observation has important consequences for the use of rhizobia. Finding methods to resuscitate this fraction may increase the quality of powder-based seed inocula.
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Registered trials
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