ArticleJournal of inflammation research2011
Transcriptional characteristics of CD4 T cells in young asthmatic children: RORC and FOXP3 axis.
Article in Journal of inflammation research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Investigating the causal association between heme oxygenase-1 and asthma: A bidirectional two-sample Mendelian randomization analysis in a European population.The World Allergy Organization journal · 2024Article
- Gut-lung axis and asthma: A historical review on mechanism and future perspective.Clinical and translational allergy · 2024Review
- Increased T-bet/GATA-3 and ROR-γt /Foxp3 Ratios in Cerebrospinal Fluid as Potential Criteria for Definite Neuro-Behçet's Disease.Journal of clinical medicine · 2022Article
- TThe Journal of allergy and clinical immunology · 2022Article
- The correlation of cytokines and sensory hypersensitivity in mild dry eye patients characterized by symptoms outweighing signs.Molecular vision · 2020Article
- Bronchial epithelial cells of young and old mice directly regulate the differentiation of Th2 and Th17.Bioscience reports · 2019Article
- Dynamics of helper CD4 T cells during acute and stable allergic asthma.Mucosal immunology · 2018Article
- Maternal exposure to farming environment protects offspring against allergic diseases by modulating the neonatal TLR-Tregs-Th axis.Clinical and translational allergy · 2018Article
- Cellular reactions to long-term volatile organic compound (VOC) exposures.Scientific reports · 2016Article
- Candidate SNP Markers of Chronopathologies Are Predicted by a Significant Change in the Affinity of TATA-Binding Protein for Human Gene Promoters.BioMed research international · 2016Article
- Association between IL-17F rs763780 polymorphism and susceptibility of asthma: a meta-analysis.International journal of clinical and experimental medicine · 2015Article
- Th17-associated cytokines as a therapeutic target for steroid-insensitive asthma.Clinical & developmental immunology · 2013Review
- The impact of vitamin D deficiency on immune T cells in asthmatic children: a case-control study.Journal of asthma and allergy · 2012Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAsthma is a chronic inflammatory disorder, hypothetically caused by autoreactive Th2 cells, whereas Th1 and regulatory T cells may confer protection. The development of Th subpopulations is dependent on the expression of lineage-specific transcription factors. PURPOSE: This study aimed to assess the balance of CD4(+) T cell populations in asthmatic children.
methodsPeripheral blood mononuclear cells (PBMC) mRNA expression was assessed in 30 asthmatic children (18 patients with mild asthma and 12 with moderate asthma). Real-time polymerase chain reaction (RT-PCR) quantified TBX21, GATA-3, RORC, FOXP3, and EBI3 mRNA expression. Intracellular cytokine expression of IL-2, IL-4, IL-10, and IFN-γ in CD4(+) T cells in asthmatic children was measured by flow cytometry. IL-6 and IL-17 cytokines were assessed in serum by enzyme-linked immunosorbent assay (ELISA).
resultsA significant increase was found in the percentage of CD4(+) and CD8(+) T cell-producing IL-4, IL-6, and IL-17. A decreased percentage of CD4(+) producing IFN-γ in asthmatic children was found. Expression of GATA-3 (Th2), retinoid-related orphan receptor C (RORC) (Th17), and EBI3 were increased in asthmatic patients compared to healthy controls. Expression of FOXP3 (Treg) and TBX21 (Th1) were decreased (P < 0.0001 and P < 0.0001) in asthmatic children. Analysis of transcription factor ratios revealed an increase in the RORC/FOXP3 (P = 0.0001), and a significant decrease of TBX21/GATA-3 (P = 0.0001) ratios in patients with asthma.
conclusionYoung asthmatics were characterized by increased IL-4 production and low IFN-γ synthesis. The increased serum IL-17 and IL-6 levels sustained an inflammatory environment in young asthmatics. The results indicate that FOXP3 and RORC mRNA expression could be associated with the sustained inflammatory process, transduced by low immune tolerance by Treg cells. The TBX21/GATA-3 and RORC/FOXP3 ratios dysregulation in asthmatics is consistent with the plasticity existing between Th1, Th17, and Treg cells during inflammation.
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