ArticleMethods in molecular biology (Clifton, N.J.)2012
Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 39 citations in OpenAlex.
- The role of epithelial membrane-associated mucin 4 in ocular surface health and corneal wound healing.Frontiers in medicine · 2025Article
- Electrospun-Reinforced Suturable Biodegradable Artificial Cornea.ACS applied bio materials · 2022Article
- Pathogenesis of keratoconus: NRF2-antioxidant, extracellular matrix and cellular dysfunctions.Experimental eye research · 2022Review
- A Mucin-Deficient Ocular Surface Mimetic Platform for Interrogating Drug Effects on Biolubrication, Antiadhesion Properties, and Barrier Functionality.ACS applied materials & interfaces · 2022Article
- Alleviation of Endoplasmic Reticulum Stress Enhances Human Corneal Epithelial Cell Viability under Hyperosmotic Conditions.International journal of molecular sciences · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Analysis of the Association between Galectin-3 Concentration in Tears and the Severity of Dry Eye Disease: A Case-Control Study.Journal of clinical medicine · 2021Article
- Graphene-Lined Porous Gelatin Glycidyl Methacrylate Hydrogels: Implications for Tissue Engineering.ACS applied nano materials · 2021Article
- CRISPR/Cas9 genome editing reveals an essential role for basigin in maintaining a nonkeratinized squamous epithelium in cornea.FASEB bioAdvances · 2021Article
- Photo-cross-linked Gelatin Glycidyl Methacrylate/N-Vinylpyrrolidone Copolymeric Hydrogel with Tunable Mechanical Properties for Ocular Tissue Engineering Applications.ACS applied bio materials · 2021Article
- Mucolytic bacteria: prevalence in various pathological diseases.World journal of microbiology & biotechnology · 2021Review
- Characterization of the rabbit conjunctiva: Effects of sulfur mustard.Experimental and molecular pathology · 2021Article
- Chimeric Claudins: A New Tool to Study Tight Junction Structure and Function.International journal of molecular sciences · 2021Article
- Differential effects of Hsp90 inhibition on corneal cells in vitro and in vivo.Experimental eye research · 2021Article
- Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.Cell adhesion & migration · 2020Article
- The O-GlcNAc modification promotes terminal differentiation of human corneal epithelial cells.Glycobiology · 2020Article
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- Endoplasmic reticulum stress promotes inflammation-mediated proteolytic activity at the ocular surface.Scientific reports · 2020Article
- Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model.Scientific reports · 2019Article
- Inflammatory Stress Causes N-Glycan Processing Deficiency in Ocular Autoimmune Disease.The American journal of pathology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Mucins of the corneal and conjunctival epithelia are necessary for the protection of the ocular surface against desiccation, pathogen access, and injury. Detection and quantification of mucins is important for the understanding of ocular surface diseases that cause impaired vision and, in advanced stages, blindness. Advances in the field of molecular biology have made it possible to study membrane mucins and their associated O-glycans in established cell culture models of human ocular surface epithelia. This chapter discusses procedures to detect and quantify mucin RNA and protein in biological samples, as well as methods to experimentally manipulate the epithelia in culture by shRNA, to understand the function of specific mucins. Example protocols are provided to evaluate the role of ocular surface mucins in mucosal barrier function and bacteria-host interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.