ArticleProtein & cell2012
A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture.
Article in Protein & cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Development of Cellular Energy Metabolism During Differentiation of Human iPSCs into Cortical Neurons.Molecular neurobiology · 2025Article
- In vitro human cell culture models in a bench-to-bedside approach to epilepsy.Epilepsia open · 2024Review
- Enhancing regenerative medicine: the crucial role of stem cell therapy.Frontiers in neuroscience · 2024Review
- Isolation, Culture, and Functional Characterization of Human Embryonic Stem Cells: Current Trends and Challenges.Stem cells international · 2018Review
- Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems.Stem cell research & therapy · 2016Article
- A decade of transcription factor-mediated reprogramming to pluripotency.Nature reviews. Molecular cell biology · 2016Review
- Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems.Reproductive biology and endocrinology : RB&E · 2015Review
- Development of a xeno-free substrate for human embryonic stem cell growth.Stem cells international · 2015Article
- CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes.Scientific reports · 2014Article
- Induced pluripotent stem cells as custom therapeutics for retinal repair: progress and rationale.Experimental eye research · 2014Review
- Article
- Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma.In vitro cellular & developmental biology. Animal · 2013Article
- Efficient generation of functional epithelial and epidermal cells from human pluripotent stem cells under defined conditions.Tissue engineering. Part C, Methods · 2013Article
- Defining synthetic surfaces for human pluripotent stem cell culture.Cell regeneration (London, England) · 2013Review
- Generation of disease-specific induced pluripotent stem cells from patients with different karyotypes of Down syndrome.Stem cell research & therapy · 2012Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While human induced pluripotent stem cells (hiPSCs) have promising applications in regenerative medicine, most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials, such as sialic acid, and potential pathogens from animal-product-containing cell culture systems. Although several xeno-free cell culture systems have been established recently, their use of human fibroblasts as feeders reduces the clinical potential of hiPSCs due to batch-to-batch variation in the feeders and time-consuming preparation processes. In this study, we have developed a xeno-free and feeder-cell-free human embryonic stem cell (hESC)/hiPSC culture system using human plasma and human placenta extracts. The system maintains the self-renewing capacity and pluripotency of hESCs for more than 40 passages. Human iPSCs were also derived from human dermal fibroblasts using this culture system by overexpressing three transcription factors-Oct4, Sox2 and Nanog. The culture system developed here is inexpensive and suitable for large scale production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.