ArticlePlanta2012
Artificial ubiquitylation is sufficient for sorting of a plasma membrane ATPase to the vacuolar lumen of Arabidopsis cells.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN inFrontiers in plant science · 2023Article
- Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking.Nature communications · 2022Article
- The Beginning of the End: Initial Steps in the Degradation of Plasma Membrane Proteins.Frontiers in plant science · 2020Review
- CesA6 and PGIP2 Endocytosis Involves Different Subpopulations of TGN-Related Endosomes.Frontiers in plant science · 2020Article
- Remove, Recycle, Degrade: Regulating Plasma Membrane Protein Accumulation.The Plant cell · 2019Review
- Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat.Scientific reports · 2017Article
- Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death.Scientific reports · 2017Article
- PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula.Plant physiology · 2016Article
- Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm.Protoplasma · 2016Article
- Molecular Composition of Plant Vacuoles: Important but Less Understood Regulations and Roles of Tonoplast Lipids.Plants (Basel, Switzerland) · 2015Review
- Plasma membrane-association of SAUL1-type plant U-box armadillo repeat proteins is conserved in land plants.Frontiers in plant science · 2014Article
- Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants.Current genetics · 2013Review
- The deubiquitinating enzyme AMSH1 and the ESCRT-III subunit VPS2.1 are required for autophagic degradation in Arabidopsis.The Plant cell · 2013Article
- Advanced proteomic analyses yield a deep catalog of ubiquitylation targets in Arabidopsis.The Plant cell · 2013Article
- Endocytic Pathways and Recycling in Growing Pollen Tubes.Plants (Basel, Switzerland) · 2013Review
- ESCRT-I mediates FLS2 endosomal sorting and plant immunity.PLoS genetics · 2013Article
- Ubiquitin initiates sorting of Golgi and plasma membrane proteins into the vacuolar degradation pathway.BMC plant biology · 2012Article
- Ubiquitin on the move: the ubiquitin modification system plays diverse roles in the regulation of endoplasmic reticulum- and plasma membrane-localized proteins.Plant physiology · 2012Review
- Regulation of plant immune receptors by ubiquitination.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorting of transmembrane proteins into the inner vesicles of multivesicular bodies for subsequent delivery to the vacuole/lysosome can be induced by attachment of a single ubiquitin or K63-linked ubiquitin chains to the cytosolic portion of the cargo in yeast and mammals. In plants, large efforts have been undertaken to elucidate the mechanisms of vacuolar trafficking of soluble proteins. Sorting of transmembrane proteins, by contrast, is still largely unexplored. As a proof of principle, that ubiquitin is involved in vacuolar sorting in plants we show that a translational fusion of a single ubiquitin to the Arabidopsis plasma membrane ATPase PMA-EGFP is sufficient to induce its endocytosis and sorting into the vacuolar lumen. Sorting of the artificial reporter is not dependent on ubiquitin chain formation, but involves ubiquitin's hydrophobic patch and can be inhibited by coexpression of a dominant-negative version of the ESCRT (endosomal sorting complex required for transport) related protein AtSKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1). Our results suggest that ubiquitin can in principle act as vacuolar sorting signal in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.