ArticleBMC genomics2012
Centromere-associated repeat arrays on Trypanosoma brucei chromosomes are much more extensive than predicted.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- First genome-wide centromere map of Trypanosoma cruzi suggests linear and 3D compartment boundaries and spatial clustering.BMC genomics · 2026Article
- Article
- Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei.Nature communications · 2025Article
- RAD51-mediated R-loop formation acts to repair transcription-associated DNA breaks driving antigenic variation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Kinetoplastid kinetochore proteins KKT2 and KKT3 have unique centromere localization domains.The Journal of cell biology · 2021Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- Identification of four unconventional kinetoplastid kinetochore proteins KKT22-25 inOpen biology · 2019Article
- Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome.Nucleic acids research · 2018Article
- Site-Specific Cleavage by Topoisomerase 2: A Mark of the Core Centromere.International journal of molecular sciences · 2018Article
- Identification of the centromeres ofEMBO reports · 2017Article
- Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity.PLoS pathogens · 2017Review
- Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe.Genome biology · 2015Article
- The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids.Nucleic acids research · 2015Article
- The epigenome of Trypanosoma brucei: a regulatory interface to an unconventional transcriptional machine.Biochimica et biophysica acta · 2014Review
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- Review
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5 authors.
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Abstract
backgroundAfrican trypanosomes belong to a eukaryotic lineage which displays many unusual genetic features. The mechanisms of chromosome segregation in these diploid protozoan parasites are poorly understood. Centromeres in Trypanosoma brucei have been localised to chromosomal regions that contain an array of ~147 bp AT-rich tandem repeats. Initial estimates from the genome sequencing project suggested that these arrays ranged from 2 - 8 kb. In this paper, we show that the centromeric repeat regions are much more extensive.
resultsWe used a long-range restriction endonuclease mapping approach to more accurately define the sizes of the centromeric repeat arrays on the 8 T. brucei chromosomes where unambiguous assembly data were available. The results indicate that the sizes of the arrays on different chromosomes vary from 20 to 120 kb. In addition, we found instances of length heterogeneity between chromosome homologues. For example, values of 20 and 65 kb were obtained for the arrays on chromosome 1, and 50 and 75 kb for chromosome 5.
conclusionsOur results show that centromeric repeat arrays on T. brucei chromosomes are more similar in size to those of higher eukaryotes than previously suspected. This information provides a firmer framework for investigating aspects of chromosome segregation and will allow epigenetic features associated with the process to be more accurately mapped.
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