ArticleMalaria journal2012
Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Synthesis, Antimalarial Activity and Molecular Dynamics Studies of Pipecolisporin: A Novel Cyclic Hexapeptide with Potent Therapeutic Potential.Molecules (Basel, Switzerland) · 2025Article
- Optimized plasmid loading of human erythrocytes for Plasmodium falciparum DNA transfections.International journal for parasitology · 2024Article
- Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum.Scientific reports · 2019Article
- Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins.Molecular biology of the cell · 2018Article
- Article
- A cross strain Plasmodium falciparum microarray optimized for the transcriptome analysis of Plasmodium falciparum patient derived isolates.Genomics data · 2016Article
- A redesigned CRISPR/Cas9 system for marker-free genome editing in Plasmodium falciparum.Parasites & vectors · 2016Article
- Article
- Plasmodium evasion of mosquito immunity and global malaria transmission: The lock-and-key theory.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum.Malaria journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of Plasmodium falciparum. Compared to standard protocols plate-based transfection requires 20-fold less DNA, transient transfection efficiency achieved is approximately seven-fold higher, whilst stable transfection success rate is above 90%. Furthermore the utility of this set of protocols to generate a knockout of the PfRH3 pseudogene, screened by whole-cell PCR, is demonstrated. The methods and tools presented here will facilitate genome-scale genetic manipulation of P. falciparum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.