Evidence map›Paper›PMID 22253979›Full record

ArticleNutrients2009

Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in Lactobacillus sakei strains.

Maria Grazia Bonomo, Maria Anna Sico, Simona Grieco, Giovanni Salzano

Abstract read
In one paragraph

Article in Nutrients, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maria Grazia BonomoDipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy. mariaanna.sico@unibas.it
Maria Anna Sico
Simona Grieco
Giovanni Salzano

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions.

Indexed as

Stress, PhysiologicalFluorescenceGene Expression Regulation, BacterialLactobacillusPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityFluorescent Differential Display PCRLactobacillus sakeistress response

Identifiers

PMID22253979
PMCPMC3257592

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.