ArticlePloS one2012
Curing of plasmid pXO1 from Bacillus anthracis using plasmid incompatibility.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- A predatory gene drive for targeted control of self-transmissible plasmids.Science advances · 2025Article
- Unveiling the impact of cryptic plasmids curing on Escherichia coli Nissle 1917: massive increase in Ag43c expression.AMB Express · 2024Article
- Genome Sequence and Phenotypic Analysis of a Protein Lysis-Negative, Attenuated Anthrax Vaccine Strain.Biology · 2023Article
- A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection ofEmerging microbes & infections · 2022Article
- Prevalence, Antibiotics Resistance and Plasmid Profiling ofMicroorganisms · 2022Article
- Predicting plasmid persistence in microbial communities by coarse-grained modeling.BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Article
- Obtaining Specific Sequence Tags forPathogens (Basel, Switzerland) · 2021Article
- Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing ofFrontiers in microbiology · 2021Article
- Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance.Frontiers in microbiology · 2020Review
- ParC, a New Partitioning Protein, Is Necessary for the Active Form of ParA FromFrontiers in microbiology · 2020Article
- Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia.BMC microbiology · 2019Article
- Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing.FEMS microbiology reviews · 2018Review
- An insight of traditional plasmid curing in Vibrio species.Frontiers in microbiology · 2015Review
- Whole Genome-Sequencing and Phylogenetic Analysis of a Historical Collection of Bacillus anthracis Strains from Danish Cattle.PloS one · 2015Article
- Antibiotic-resistant vibrios in farmed shrimp.BioMed research international · 2015Article
- Occurrence and Antibiotic Resistance of Vibrio parahaemolyticus from Shellfish in Selangor, Malaysia.Frontiers in microbiology · 2015Article
- Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in Malaysia.Frontiers in microbiology · 2015Article
- Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria.Journal of bacteriology · 2014Article
- Evolution and dynamics of megaplasmids with genome sizes larger than 100 kb in the Bacillus cereus group.BMC evolutionary biology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or growth at elevated temperatures) can induce mutations in the host chromosomal DNA, we developed a specific and reliable method to eliminate pXO1 from B. anthracis using plasmid incompatibility. Three putative replication origins of pXO1 were inserted into a temperature-sensitive plasmid to generate three incompatible plasmids. One of the three plasmids successfully eliminated the large plasmid pXO1 from B. anthracis vaccine strain A16R and wild type strain A16. These findings provided additional information about the replication/partitioning of pXO1 and demonstrated that introducing a small incompatible plasmid can generate plasmid-cured strains of B. anthracis without inducing spontaneous mutations in the host chromosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.