ArticlePloS one2012
Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- The mycobacterial nucleoid-associated protein NapM exhibits stress-induced septal localization and modulates cell envelope gene expression.Microbiology spectrum · 2026Article
- Exposure to stressors and antimicrobials induces cell-autonomous ultrastructural heterogeneity of an intracellular bacterial pathogen.Frontiers in cellular and infection microbiology · 2022Article
- Measurable genomic changes inJournal of bacteriology · 2021Article
- Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii.Arhiv za higijenu rada i toksikologiju · 2020Article
- Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages.PLoS pathogens · 2019Article
- Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes.Journal of visualized experiments : JoVE · 2018Article
- Co-culture models illustrate the digestion of Gemmata spp. by phagocytes.Scientific reports · 2018Article
- Buruli Ulcer, a Prototype for Ecosystem-Related Infection, Caused by Mycobacterium ulcerans.Clinical microbiology reviews · 2018Review
- Interaction Between Methicillin-Resistant Staphylococcus aureus (MRSA) and Acanthamoeba polyphaga.Current microbiology · 2017Article
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria.Frontiers in microbiology · 2014Article
- Mycobacterium gilvum illustrates size-correlated relationships between mycobacteria and Acanthamoeba polyphaga.Applied and environmental microbiology · 2013Article
- Antimicrobial efflux pumps and Mycobacterium tuberculosis drug tolerance: evolutionary considerations.Current topics in microbiology and immunology · 2013Article
- Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay.Scientific reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacterium smegmatis is a rapidly-growing mycobacterium causing rare opportunistic infections in human patients. It is present in soil and water environments where free-living amoeba also reside, but data regarding M. smegmatis-amoeba relationships have been contradictory from mycobacteria destruction to mycobacteria survival. METHODOLOGY/PRINCIPAL
findingsUsing optic and electron microscopy and culture-based microbial enumeration we investigated the ability of M. smegmatis mc(2) 155, M. smegmatis ATCC 19420(T) and M. smegmatis ATCC 27204 organisms to survive into Acanthamoeba polyphaga trophozoites and cysts. We observed that M. smegmatis mycobacteria penetrated and survived in A. polyphaga trophozoites over five-day co-culture resulting in amoeba lysis and the release of viable M. smegmatis mycobacteria without amoebal cyst formation. We further observed that amoeba-co-culture, and lysed amoeba and supernatant and pellet, significantly increased five-day growth of the three tested M. smegmatis strains, including a four-fold increase in intra-amoebal growth. CONCLUSIONS/SIGNIFICANCE: Amoebal co-culture increases the growth of M. smegmatis resulting in amoeba killing by replicating M. smegmatis mycobacteria. This amoeba-M. smegmatis co-culture system illustrates an unusual paradigm in the mycobacteria-amoeba interactions as mycobacteria have been mainly regarded as amoeba-resistant organisms. Using these model organisms, this co-culture system could be used as a simple and rapid model to probe mycobacterial factors implicated in the intracellular growth of mycobacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.