ArticleThe Plant cell2012
Routes to the tonoplast: the sorting of tonoplast transporters in Arabidopsis mesophyll protoplasts.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 110 citations in OpenAlex.
- Vacuolar zinc transporter TaMTP1 safeguards wheat fertility and regulates grain zinc allocation via stem-mediated sequestration.The Plant journal : for cell and molecular biology · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- The Vacuolar Inositol Transporter BvINT1;1 Contributes to Raffinose Biosynthesis and Reactive Oxygen Species Scavenging During Cold Stress in Sugar Beet.Plant, cell & environment · 2025Article
- The Phytophthora parasitica effector AVH195 interacts with ATG8, attenuates host autophagy, and promotes biotrophic infection.BMC biology · 2024Article
- Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator.Nature plants · 2023Article
- Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.The Plant cell · 2022Article
- Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.Cells · 2022Review
- Molecular mechanisms of endomembrane trafficking in plants.The Plant cell · 2022Review
- Engineering cellular metabolite transport for biosynthesis of computationally predicted tropane alkaloid derivatives in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple.BMC plant biology · 2020Article
- MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-wide identification, expression, and association analysis of the monosaccharide transporter (MST) gene family in peanut (3 Biotech · 2020Article
- AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Polar Localization of the Borate Exporter BOR1 Requires AP2-Dependent Endocytosis.Plant physiology · 2019Article
- A Tonoplast-Associated Calcium-Signaling Module Dampens ABA Signaling during Stomatal Movement.Plant physiology · 2018Article
- The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization.Plant physiology · 2018Article
- The Tonoplastic Inositol Transporter INT1 FromFrontiers in plant science · 2018Article
- Update on adaptor protein-3 in Arabidopsis.Plant signaling & behavior · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuoles perform a multitude of functions in plant cells, including the storage of amino acids and sugars. Tonoplast-localized transporters catalyze the import and release of these molecules. The mechanisms determining the targeting of these transporters to the tonoplast are largely unknown. Using the paralogous Arabidopsis thaliana inositol transporters INT1 (tonoplast) and INT4 (plasma membrane), we performed domain swapping and mutational analyses and identified a C-terminal di-leucine motif responsible for the sorting of higher plant INT1-type transporters to the tonoplast in Arabidopsis mesophyll protoplasts. We demonstrate that this motif can reroute other proteins, such as INT4, SUCROSE TRANSPORTER2 (SUC2), or SWEET1, to the tonoplast and that the position of the motif relative to the transmembrane helix is critical. Rerouted INT4 is functionally active in the tonoplast and complements the growth phenotype of an int1 mutant. In Arabidopsis plants defective in the β-subunit of the AP-3 adaptor complex, INT1 is correctly localized to the tonoplast, while sorting of the vacuolar sucrose transporter SUC4 is blocked in cis-Golgi stacks. Moreover, we demonstrate that both INT1 and SUC4 trafficking to the tonoplast is sensitive to brefeldin A. Our data show that plants possess at least two different Golgi-dependent targeting mechanisms for newly synthesized transporters to the tonoplast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.