ArticleAntimicrobial agents and chemotherapy2012
Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Approaches for Studying Context Specificity of Translation Inhibitor Action.International journal of molecular sciences · 2026Review
- Article
- Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.Scientific reports · 2024Article
- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
- Antibiotics that affect translation can antagonize phage infectivity by interfering with the deployment of counter-defenses.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Flow-Seq Evaluation of Translation Driven by a Set of NaturalInternational journal of molecular sciences · 2022Article
- Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project.Antibiotics (Basel, Switzerland) · 2022Article
- Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert.Journal of pharmaceutical analysis · 2021Article
- Translation at first sight: the influence of leading codons.Nucleic acids research · 2020Article
- Influence of the spacer region between the Shine-Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli.Microbial biotechnology · 2020Article
- 2-Pyrazol-1-yl-thiazole derivatives as novel highly potent antibacterials.The Journal of antibiotics · 2019Article
- Monitoring of Antimicrobial Drug Chloramphenicol Release from Electrospun Nano- and Microfiber Mats Using UV Imaging and Bacterial Bioreporters.Pharmaceutics · 2019Article
- Synthesis and evaluation of biological activity of benzoxaborole derivatives of azithromycin.The Journal of antibiotics · 2019Article
- Mitochondria-targeted antioxidants as highly effective antibiotics.Scientific reports · 2017Article
- Application of sorting and next generation sequencing to study 5΄-UTR influence on translation efficiency in Escherichia coli.Nucleic acids research · 2017Article
- Sorting Out Antibiotics' Mechanisms of Action: a Double Fluorescent Protein Reporter for High-Throughput Screening of Ribosome and DNA Biosynthesis Inhibitors.Antimicrobial agents and chemotherapy · 2016Article
- Techniques for Screening Translation Inhibitors.Antibiotics (Basel, Switzerland) · 2016Review
- Article
- Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.Molecular cell · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.