Evidence map›Paper›PMID 22252335›Full record

ArticleFish physiology and biochemistry2012

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis).

Eric D H Durieux, Richard E Connon, Inge Werner, Leandro S D'Abronzo, Patrick S Fitzgerald, Jimmy L Spearow, David J Ostrach

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In one paragraph

Article in Fish physiology and biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Eric D H DurieuxPathobiology, Conservation and Population Biology Laboratory, John Muir Institute of the Environment, Center for Watershed Sciences, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA. durieux@univ-corse.fr.
Richard E ConnonAquatic Toxicology Laboratory, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, University of California, Davis, CA, 95616, USA.
Inge WernerAquatic Toxicology Laboratory, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, University of California, Davis, CA, 95616, USA.
Leandro S D'AbronzoAquatic Toxicology Laboratory, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, University of California, Davis, CA, 95616, USA.
Patrick S FitzgeraldPathobiology, Conservation and Population Biology Laboratory, John Muir Institute of the Environment, Center for Watershed Sciences, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Jimmy L SpearowPathobiology, Conservation and Population Biology Laboratory, John Muir Institute of the Environment, Center for Watershed Sciences, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
David J OstrachPathobiology, Conservation and Population Biology Laboratory, John Muir Institute of the Environment, Center for Watershed Sciences, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
University of California, Davis · USUniversity of California System · USUniversité de Corse Pascal Paoli · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The striped bass (Morone saxatilis) supports a valuable recreational fishery and is among the most important piscivorous fish of the San Francisco Estuary. This species has suffered a significant decline in numbers over the past decades, and there is indication that contaminants are important contributors. Polycyclic aromatic hydrocarbons (PAHs) and polyhalogenated aromatic hydrocarbons (PHAHs) including PCBs and dioxins are widespread in the estuary, they typically bioaccumulate through trophic levels, reaching highest levels in top predators and are known to affect the fish health and development. The aim of this study was to investigate the dynamics of cytochrome P4501A (Cyp1a) induction simultaneously at different levels of biological organization (RNA transcription and protein synthesis) as a biomarker of exposure to PAHs and PHAHs. We utilized β-naphthoflavone (BNF) as a model PAH to induce Cyp1a responses in juvenile striped bass in both dose-response and time-response assessments and determined Cyp1a mRNA and protein levels. Significant responses were measured in both systems at 10 mg ΒΝF kg⁻¹, a concentration used for time-response studies. Messenger RNA levels peaked at 6 h post-injection, while protein levels increased progressively with time, significantly peaking at 96 h post-injection; both remaining elevated throughout the duration of the test (8 days). Our data suggest that rapid induction of gene transcription following exposure and subsequent cumulative protein synthesis could provide a useful means of identifying temporal variants in exposure to Cyp1a inducers in Morone saxatilis. The potential application of this combined Cyp1a gene and protein biomarker in this species for field studies is discussed.

Indexed as

AnimalsBassbeta-NaphthoflavoneBiomarkersCytochrome P-450 Enzyme SystemDose-Response Relationship, DrugEnvironmental MonitoringFish ProteinsPolycyclic Aromatic HydrocarbonsRNA, MessengerTime FactorsXenobioticsbeta-NaphthoflavoneBiomarkersCytochrome P-450 Enzyme SystemFish ProteinsPolycyclic Aromatic HydrocarbonsRNA, MessengerXenobiotics

Identifiers

PMID22252335
OpenAlexW2019186731

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.